Metal-Ion-Responsive Fluorescent Probes for Two-Photon Excitation Microscopy

被引：163

作者：

Sumalekshmy, S.

Fahrni, Christoph J. ^{[1
]}

机构：

[1] Georgia Inst Technol, Sch Chem & Biochem, Atlanta, GA 30332 USA

来源：

CHEMISTRY OF MATERIALS | 2011年 / 23卷 / 03期

基金：

美国国家卫生研究院;

关键词：

INTRAMOLECULAR PROTON-TRANSFER; ABSORPTION CROSS-SECTIONS; IN-VIVO; FUSION PROTEINS; MULTIPHOTON MICROSCOPY; ABSORBING MATERIALS; ACIDIC VESICLES; CALCIUM WAVES; ZINC; DESIGN;

D O I：

10.1021/cm1021905

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Metal-ion-responsive fluorescent probes are powerful tools for visualizing labile metal ion pools in live cells. To take full advantage of the benefits offered by two-photon excitation microscopy, including increased depth penetration, reduced phototoxicity, and intrinsic 3D capabilities, the photophysical properties of the probes must be optimized for nonlinear excitation. This review summarizes the challenges associated with the design of two-photon excitable fluorescent probes and labels and offers an overview on recent efforts in developing selective and sensitive reagents for the detection of metal ions in biological systems.

引用

页码：483 / 500

页数：18

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