Interaction of the adenovirus major core protein precursor, pVII, with the viral DNA packaging machinery

被引:22
|
作者
Zhang, W [1 ]
Arcos, R [1 ]
机构
[1] Univ Texas, Hlth Sci Ctr, Dept Pharmacol, San Antonio, TX 78229 USA
关键词
adenovirus; assembly; packaging; encapsidation; core; pVll; IVa2;
D O I
10.1016/j.virol.2005.01.048
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Adenovirus is one of the well-studied double-stranded DNA viruses. However, the mechanisms of its DNA packaging and virion assembly are still not fully understood. One of the unique features of adenovirus is that the unpackaged viral DNA is associated with core protein pVII. Packaging of viral DNA bound with proteins has not been reported from other viruses. To characterize how viral DNA bound with protein pVII is packaged, we performed experiments to see if protein pVII interacts with the known DNA packaging proteins or the packaging sequence. Our results demonstrated that protein pVII interacted with the viral IVa2 and L1 52/55 kDa proteins, which are the known viral DNA packaging proteins. Furthermore, our protein-DNA binding experiments demonstrated that the IVa2 protein mediates the specific interaction with the packaging sequence, whereas protein pVII and the L1 52/55 kDa protein bind to DNA non-specifically. Although the non-specific binding of protein pVII and the L1 52/55 kDa protein do not appear to affect the specific binding of the IVa2 protein to the packaging sequence, and the specific binding of the IVa2 protein does not appear to block the bindings of protein pVII and the L1 52/55 kDa protein to the packaging sequence, the possibility of a cooperative binding among the IVa2 protein, the L1 52/55 kDa protein and protein pVII on the packaging sequence needs to be further determined. In summary, the results indicate that the assembly of the DNA packaging initiation complex may be mediated by the specific interaction of the IVa2 protein with the packaging sequence and other viral proteins, such as protein pVII and the L1 52/55 kDa protein. (c) 2005 Elsevier Inc. All rights reserved.
引用
收藏
页码:194 / 202
页数:9
相关论文
共 50 条
  • [21] DNA sequence analysis of an avian adenovirus terminal protein precursor
    McCoy, RJ
    Sheppard, M
    VIRUS GENES, 1997, 14 (03) : 245 - 249
  • [22] IDENTIFICATION OF THE GENE CODING FOR THE PRECURSOR OF ADENOVIRUS CORE PROTEIN-X
    WEBER, JM
    ANDERSON, CW
    JOURNAL OF VIROLOGY, 1988, 62 (05) : 1741 - 1745
  • [23] ADENOVIRUS CHROMATIN-I - ISOLATION AND CHARACTERIZATION OF MAJOR CORE PROTEIN-VII AND PRECURSOR PRO-VII
    SUNG, MT
    LISCHWE, MA
    RICHARDS, JC
    HOSOKAWA, K
    JOURNAL OF BIOLOGICAL CHEMISTRY, 1977, 252 (14) : 4981 - 4987
  • [24] Functional interaction of the adenovirus IVa2 protein with adenovirus type 5 packaging sequences
    Ostapchuk, P
    Yang, JH
    Auffarth, E
    Hearing, P
    JOURNAL OF VIROLOGY, 2005, 79 (05) : 2831 - 2838
  • [25] THE OCT-1 POU DOMAIN STIMULATES ADENOVIRUS DNA-REPLICATION BY A DIRECT INTERACTION BETWEEN THE VIRAL PRECURSOR TERMINAL PROTEIN-DNA POLYMERASE COMPLEX AND THE POU HOMEODOMAIN
    COENJAERTS, FEJ
    VANOOSTERHOUT, JAWM
    VANDERVLIET, PC
    EMBO JOURNAL, 1994, 13 (22): : 5401 - 5409
  • [26] ANALYSIS OF THE INTERACTION BETWEEN DNA AND MAJOR CORE PROTEIN IN ADENOVIRUS CHROMATIN BY CIRCULAR-DICHROISM AND ULTRAVIOLET-LIGHT INDUCED CROSS-LINKING
    SATO, K
    HOSOKAWA, K
    JOURNAL OF BIOCHEMISTRY, 1984, 95 (04): : 1031 - 1039
  • [27] Adenovirus major core protein condenses DNA in clusters and bundles, modulating genome release and capsid internal pressure
    Martin-Gonzalez, Natalia
    Hernando-Perez, Mercedes
    Condezo, Gabriela N.
    Perez-Illana, Marta
    Siber, Antonio
    Reguera, David
    Ostapchuk, Philomena
    Hearing, Patrick
    San Martin, Carmen
    de Pablo, Pedro J.
    NUCLEIC ACIDS RESEARCH, 2019, 47 (17) : 9231 - 9242
  • [28] The Adenovirus L4-33K Protein Regulates both Late Gene Expression Patterns and Viral DNA Packaging
    Wu, Kai
    Guimet, Diana
    Hearing, Patrick
    JOURNAL OF VIROLOGY, 2013, 87 (12) : 6739 - 6747
  • [29] Distribution of DNA-condensing protein complexes in the adenovirus core
    Perez-Berna, Ana J.
    Marion, Sanjin
    Javier Chichon, F.
    Fernandez, Jose J.
    Winkler, Dennis C.
    Carrascosa, Jose L.
    Steven, Alasdair C.
    Siber, Antonio
    San Martin, Carmen
    NUCLEIC ACIDS RESEARCH, 2015, 43 (08) : 4274 - 4283
  • [30] CHARACTERIZATION OF THE CHYMOTRYPTIC CORE OF THE ADENOVIRUS DNA-BINDING PROTEIN
    TSERNOGLOU, D
    TSUGITA, A
    TUCKER, AD
    VANDERVLIET, PC
    FEBS LETTERS, 1985, 188 (02) : 248 - 252