Insect-resistant transgenic Pinus radiata

被引：38
|
作者：
Grace, LJ [1 ]
Charity, JA [1 ]
Gresham, B [1 ]
Kay, N [1 ]
Walter, C [1 ]
机构：
[1] New Zealand Forest Res Inst Ltd, Rotorua, New Zealand
来源：
PLANT CELL REPORTS | 2005年 / 24卷 / 02期
关键词：
biolistic transformation; embryogenic tissue; cry1Ac; conifers;
D O I：
10.1007/s00299-004-0912-x
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Transgenic radiata pine (Pinus radiata D. Don) plants containing a Bacillus thuringiensis (Bt) toxin gene, crylAc, were produced by means of biolistic transformation of embryogenic tissue. Using the selectable marker gene nptII and corresponding geneticin selection, 20 independent transgenic lines from five genotypes were established. Over 200 plants regenerated from ten transgenic lines were successfully transferred to soil. The integration and expression of the introduced genes in transgenic tissue and/or plants were confirmed by PCR, Southern hybridisation and neomycin phosphotransferase II (NPTII) and Bt ELISA assays. Bioassays with larvae of the painted apple moth, Teia anartoides, demonstrated that transgenic plants displayed variable levels of resistance to insect damage, with one transgenic line being highly resistant to feeding damage.
引用
收藏
页码：103 / 111
页数：9
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