Epitope tagging of endogenous proteins for genome-wide ChIP-chip studies

被引：24

作者：

Zhang, Xiaodong ^{[1
,2
]}

Guo, Chunguang ^{[3
]}

Chen, Yueting ^{[1
,2
]}

Shulha, Hennady P. ^{[4
]}

Schnetz, Michael P. ^{[1
,2
]}

LaFramboise, Thomas ^{[1
,2
]}

Bartels, Cynthia F. ^{[1
,2
]}

Markowitz, Sanford ^{[5
,6
,7
,8
]}

Weng, Zhiping ^{[4
,9
]}

Scacheri, Peter C. ^{[1
,2
]}

Wang, Zhenghe ^{[1
,2
,10
]}

机构：

[1] Case Western Reserve Univ, Dept Genet, Cleveland, OH 44106 USA

[2] Case Western Reserve Univ, Case Comprehens Canc Ctr, Cleveland, OH 44106 USA

[3] Case Western Reserve Univ, Dept Mol Biol & Microbiol, Cleveland, OH 44106 USA

[4] Boston Univ, Dept Biomed Engn, Boston, MA 02215 USA

[5] Case Western Reserve Univ, Dept Med, Cleveland, OH 44106 USA

[6] Case Western Reserve Univ, Ireland Canc Ctr, Cleveland, OH 44106 USA

[7] Univ Hosp Cleveland, Cleveland, OH 44106 USA

[8] Howard Hughes Med Inst, Cleveland, OH 44106 USA

[9] Boston Univ, Bioinformat Program, Boston, MA 02215 USA

[10] Cleveland Clin Fdn, Genom Med Inst, Cleveland, OH 44195 USA

来源：

NATURE METHODS | 2008年 / 5卷 / 02期

关键词：

D O I：

10.1038/NMETH1170

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We developed a strategy to introduce epitope tag-encoding DNA into endogenous loci by homologous recombination-mediated 'knock-in'. The tagging method is straightforward, can be applied to many loci and several human somatic cell lines, and can facilitate many functional analyses including western blot, immunoprecipitation, immunofluorescence and chromatin immunoprecipitation-microarray (ChIP-chip). The knock-in approach provides a general solution for the study of proteins to which antibodies are substandard or not available.

引用

页码：163 / 165

页数：3

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