Epitope tagging of endogenous proteins for genome-wide ChIP-chip studies

被引：24

作者：

Zhang, Xiaodong ^{[1
,2
]}

Guo, Chunguang ^{[3
]}

Chen, Yueting ^{[1
,2
]}

Shulha, Hennady P. ^{[4
]}

Schnetz, Michael P. ^{[1
,2
]}

LaFramboise, Thomas ^{[1
,2
]}

Bartels, Cynthia F. ^{[1
,2
]}

Markowitz, Sanford ^{[5
,6
,7
,8
]}

Weng, Zhiping ^{[4
,9
]}

Scacheri, Peter C. ^{[1
,2
]}

Wang, Zhenghe ^{[1
,2
,10
]}

机构：

[1] Case Western Reserve Univ, Dept Genet, Cleveland, OH 44106 USA

[2] Case Western Reserve Univ, Case Comprehens Canc Ctr, Cleveland, OH 44106 USA

[3] Case Western Reserve Univ, Dept Mol Biol & Microbiol, Cleveland, OH 44106 USA

[4] Boston Univ, Dept Biomed Engn, Boston, MA 02215 USA

[5] Case Western Reserve Univ, Dept Med, Cleveland, OH 44106 USA

[6] Case Western Reserve Univ, Ireland Canc Ctr, Cleveland, OH 44106 USA

[7] Univ Hosp Cleveland, Cleveland, OH 44106 USA

[8] Howard Hughes Med Inst, Cleveland, OH 44106 USA

[9] Boston Univ, Bioinformat Program, Boston, MA 02215 USA

[10] Cleveland Clin Fdn, Genom Med Inst, Cleveland, OH 44195 USA

来源：

NATURE METHODS | 2008年 / 5卷 / 02期

关键词：

D O I：

10.1038/NMETH1170

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We developed a strategy to introduce epitope tag-encoding DNA into endogenous loci by homologous recombination-mediated 'knock-in'. The tagging method is straightforward, can be applied to many loci and several human somatic cell lines, and can facilitate many functional analyses including western blot, immunoprecipitation, immunofluorescence and chromatin immunoprecipitation-microarray (ChIP-chip). The knock-in approach provides a general solution for the study of proteins to which antibodies are substandard or not available.

引用

页码：163 / 165

页数：3

共 50 条

[1] Epitope tagging of endogenous proteins for genome-wide ChIP-chip studies
Zhang X.
Guo C.
Chen Y.
Shulha H.P.
Schnetz M.P.
LaFramboise T.
Bartels C.F.
Markowitz S.
Weng Z.
Scacheri P.C.
Wang Z.
Nature Methods, 2008, 5 (2) : 163 - 165
[2] Genome-Wide Occupancy Analysis by ChIP-chip and ChIP-Seq
Hao, Hong
RETINAL DEGENERATIVE DISEASES, 2012, 723 : 753 - 759
[3] ChIP-chip comes of age for genome-wide functional analysis
Wu, Jiejun
Smith, Laura T.
Plass, Christoph
Huang, Tim H-M.
CANCER RESEARCH, 2006, 66 (14) : 6899 - 6902
[4] Poisson approximation for significance in genome-wide ChIP-chip tiling arrays
Zhang, Yu
BIOINFORMATICS, 2008, 24 (24) : 2825 - 2831
[5] Comparing genome-wide chromatin profiles using ChIP-chip or ChIP-seq
Johannes, Frank
Wardenaar, Rene
Colome-Tatche, Maria
Mousson, Florence
de Graaf, Petra
Mokry, Michal
Guryev, Victor
Timmers, H. Th. Marc
Cuppen, Edwin
Jansen, Ritsert C.
BIOINFORMATICS, 2010, 26 (08) : 1000 - 1006
[6] ChIP-chip: considerations for the design, analysis, and application of genome-wide chromatin immunoprecipitation experiments
Buck, MJ
Lieb, JD
GENOMICS, 2004, 83 (03) : 349 - 360
[7] Genome-wide analysis of histone modifications by ChIP-chip to identify silenced genes in gastric cancer
Zhu, Xinjiang
Liu, Jian
Xu, Xiaoyang
Zhang, Chundong
Dai, Dongqiu
ONCOLOGY REPORTS, 2015, 33 (05) : 2567 - 2574
[8] Genome-Wide Modeling of Transcription Preinitiation Complex Disassembly Mechanisms using ChIP-chip Data
Samorodnitsky, Eric
Pugh, B. Franklin
PLOS COMPUTATIONAL BIOLOGY, 2010, 6 (04)
[9] Using ChIP-chip and ChIP-seq to study the regulation of gene expression: Genome-wide localization studies reveal widespread regulation of transcription elongation
Gilchrist, Daniel A.
Fargo, David C.
Adelman, Karen
METHODS, 2009, 48 (04) : 398 - 408
[10] ENCODE and ChIP-chip in the genome era
Boguski, MS
GENOMICS, 2004, 83 (03) : 347 - 348

← 1 2 3 4 5 →