Rapid extraction of DNA from Escherichia coli and Cryptosporidium parvum for use in PCR

被引：20
|
作者：
Higgins, JA [1 ]
Jenkins, MC [1 ]
Shelton, DR [1 ]
Fayer, R [1 ]
Karns, JS [1 ]
机构：
[1] USDA ARS, Beltsville, MD 20705 USA
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2001年 / 67卷 / 11期
关键词：
D O I：
10.1128/AEM.67.11.5321-5324.2001
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The Xtra Amp tube, Isocode paper, Instagene matrix, and PrepMan matrix methods were evaluated for their ability to rapidly extract PCR-quality DNAs from Escherichia coli O157:H7 and Cryptosporidium parvum. All ability methods provided satisfactory DNA from E. coli, and the Xtra Amp and Instagene reagents provided satisfactory DNA from C. parvum.
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页码：5321 / 5324
页数：4
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