Fiber-Optic Immunosensor for Detection of Crimean-Congo Hemorrhagic Fever IgG Antibodies in Patients

被引:34
|
作者
Algaar, Fairoz [1 ]
Eltzov, Evgeni [2 ,3 ]
Vdovenko, Marina M. [4 ]
Sakharov, Ivan Yu. [4 ]
Fajs, Luka [3 ]
Weidmann, Manfred [5 ]
Mirazimi, Ali [6 ,7 ]
Marks, Robert S. [1 ,2 ,3 ,8 ,9 ,10 ]
机构
[1] Ben Gurion Univ Negev, Dept Environm Engn, IL-84105 Beer Sheva, Israel
[2] Ben Gurion Univ Negev, Dept Biotechnol Engn, IL-84105 Beer Sheva, Israel
[3] Nanyang Technol Univ, Sch Mat Sci & Engn, Singapore 639798, Singapore
[4] Moscow MV Lomonosov State Univ, Dept Chem, Moscow 119991, Russia
[5] Univ Stirling, Inst Aquaculture, Stirling FK9 4LA, Scotland
[6] Publ Hlth Agcy Sweden, Stockholm, Sweden
[7] Karolinska Inst, Stockholm, Sweden
[8] Natl Vet Inst, S-75007 Uppsala, Sweden
[9] Ben Gurion Univ Negev, Natl Inst Biotechnol Negev, IL-84105 Beer Sheva, Israel
[10] Ben Gurion Univ Negev, Ilse Katz Ctr Meso & Nanoscale Sci & Technol, IL-84105 Beer Sheva, Israel
关键词
VIRUS; CHEMILUMINESCENCE; TRANSMISSION; PEROXIDASE; HUMANS;
D O I
10.1021/acs.analchem.5b01728
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Crimean-Congo hemorrhagic fever (CCHF) is a severe viral disease with high fatality rate. CCHF virus is endemic in parts of Africa, Asia, the Middle East, and southeastern Europe. Rapid diagnostics of CCHF is vital for appropriate clinical management and prevention of secondary spread from human-to-human. Currently, diagnostics relies on real-time RT-PCR and antibody or antigen detection using ELISA. These methods require trained personnel and expensive equipment and are not appropriate for point-of-care (POC) diagnostics. Furthermore, there are no POC assays available for CCHF. We developed a fiber-optic biosensor for the detection of CCHF IgG antibodies. In order to improve sensitivity, we optimized both the bioreceptor immobilization protocol and the chemiluminescence substrate formulation. The resulting protocol showed a 100-fold greater sensitivity for detection of CCHF antibodies. Finally, we evaluated the fiber-optic biosensor with two CCHF patient sera. We showed that the fiber-optic biosensor is 10-times more sensitive than colorimetric ELISA and is able to detect both patients with high and low levels of IgG antibodies. We believe that the fiber-optic biosensor is a suitable alternative to ELISA as it is much more sensitive and makes it possible to detect a small amount of antibodies at an early stage of infection and can be integrated as a point-of-care diagnostic system of CCHF.
引用
收藏
页码:8394 / 8398
页数:5
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