Study of optimal conditions for growth and osteogenic differentiation of dental pulp stem cells based on glucose and serum content

被引:5
|
作者
Bhandi, Shilpa [1 ]
Alkahtani, Ahmed [2 ]
Mashyakhy, Mohammed [1 ]
Baeshen, Hosam Ali [3 ]
Mustafa, Mohammed [4 ]
Chohan, Hitesh [1 ]
Boreak, Nezar [1 ]
Patil, Shankargouda [5 ]
机构
[1] Jazan Univ, Coll Dent, Dept Restorat Dent Sci, Jazan, Saudi Arabia
[2] King Saud Univ, Coll Dent, Dept Restorat Dent Sci, Riyadh, Saudi Arabia
[3] King Abdulaziz Univ, Coll Dent, Dept Orthodont, Jeddah, Saudi Arabia
[4] Prince Sattam Bin Abdulaziz Univ, Coll Dent, Dept Conservat Dent Sci, Al Kharj, Saudi Arabia
[5] Jazan Univ, Coll Dent, Dept Maxillofacial Surg & Diagnost Sci, Div Oral Pathol, Jazan, Saudi Arabia
关键词
Dental pulp stem cells; Defined culture conditions; Differentiation; Mesenchymal stem cells;
D O I
10.1016/j.sjbs.2021.06.101
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Dental pulp stem cells (DPSCs) have shown promising characteristics in terms of their proliferation and osteogenic differentiation potential, which could be of greater benefit in regenerative dentistry. However, obstacles remain in the in vitro cultivation of DPSCs, which significantly affect their growth and differentiating ability. Therefore in this study, we demonstrated the growth and osteogenic differentiation of DPSCs in the presence of media containing different combinations of serum and glucose to get an optimized combination of both. DPSCs were cultured in media containing combinations of low glucose (LG), low serum (LS), high glucose (HG), and high serum (HS). The proliferation and osteogenic differentiation were assessed in DPSCs cultured with these different combinations of culture conditions. High glucose high serum condition significantly inhibited the proliferation of DPSCs and also affected their clonogenic potential, as evidenced by colony-forming units. Irrespective of the serum content, high glucose in the media also decreased the osteogenic potential of DPSCs confirmed by functional staining, and downregulation of osteogenesis-related genes. High glucose content in the culture media affects the growth and differentiation potential of the DPSCs. Hence, the culture conditions for the DPSCs should be reconsidered to utilize their maximum potential. (c) 2021 The Author(s). Published by Elsevier B.V. on behalf of King Saud University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
引用
收藏
页码:6359 / 6364
页数:6
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