Establishment of a novel equine cell line for isolation and propagation of equine herpesviruses

被引：20
|
作者：
Maeda, Ken
Yasumoto, Shigeru
Tsuruda, Akari
Andoh, Kiyohiko
Kai, Kazushige
Otoi, Takeshige
Matsumura, Tornio
机构：
[1] Yamaguchi Univ, Fac Agr, Lab Vet Microbiol, Yamaguchi 7538515, Japan
[2] Kanagawa Canc Ctr, Res Inst, Mol Cell Biol Lab, Yokohama, Kanagawa 2410815, Japan
[3] Yamaguchi Univ, United Grad Sch Vet Sci, Yamaguchi 7538515, Japan
[4] Epizoot Res Ctr, Gluck Equine Res Ctr, Mol Biol Div, Tochigi 3290412, Japan
来源：
JOURNAL OF VETERINARY MEDICAL SCIENCE | 2007年 / 69卷 / 09期
关键词：
cell line; equine; equine herpesvirus;
D O I：
10.1292/jvms.69.989
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
In the present study, an equine-derived cell line was established by transfecting primary fetal horse kidney (FHK) cells with expression plasmid encoding simian virus 40 (SV40) large T antigen and then cloning them by limiting dilution. The cloned cell line, named FHK-Tcl3, grew well and could be propagated over 30 times by splitting them 1:3. Equine herpesvirus (EHV)-1 and EHV-4 replicated well in FHK-Tcl3. EHV-2 and EHV-4 were isolated from samples collected from horses in the field using FHK-Tcl3, and EHV-3 also propagated in FHK-Tcl3. These results indicated that this novel cell line, FHK-Tcl3, can be used for isolation and propagation of equine herpesviruses.
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页码：989 / 991
页数：3
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