Establishment of a novel equine cell line for isolation and propagation of equine herpesviruses

被引：20

作者：

Maeda, Ken

Yasumoto, Shigeru

Tsuruda, Akari

Andoh, Kiyohiko

Kai, Kazushige

Otoi, Takeshige

Matsumura, Tornio

机构：

[1] Yamaguchi Univ, Fac Agr, Lab Vet Microbiol, Yamaguchi 7538515, Japan

[2] Kanagawa Canc Ctr, Res Inst, Mol Cell Biol Lab, Yokohama, Kanagawa 2410815, Japan

[3] Yamaguchi Univ, United Grad Sch Vet Sci, Yamaguchi 7538515, Japan

[4] Epizoot Res Ctr, Gluck Equine Res Ctr, Mol Biol Div, Tochigi 3290412, Japan

来源：

JOURNAL OF VETERINARY MEDICAL SCIENCE | 2007年 / 69卷 / 09期

关键词：

cell line; equine; equine herpesvirus;

D O I：

10.1292/jvms.69.989

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

In the present study, an equine-derived cell line was established by transfecting primary fetal horse kidney (FHK) cells with expression plasmid encoding simian virus 40 (SV40) large T antigen and then cloning them by limiting dilution. The cloned cell line, named FHK-Tcl3, grew well and could be propagated over 30 times by splitting them 1:3. Equine herpesvirus (EHV)-1 and EHV-4 replicated well in FHK-Tcl3. EHV-2 and EHV-4 were isolated from samples collected from horses in the field using FHK-Tcl3, and EHV-3 also propagated in FHK-Tcl3. These results indicated that this novel cell line, FHK-Tcl3, can be used for isolation and propagation of equine herpesviruses.

引用

页码：989 / 991

页数：3

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