Modelling and simulation of the adsorption of the lipase from Geotrichum sp on hydrophobic interaction columns

被引：8
|
作者：
Mendieta-Taboada, O
Kamimura, ES
Maugeri, F
机构：
[1] Univ Estadual Campinas, Sch Food Engn, DEA, BR-13083970 Campinas, SP, Brazil
[2] Univ Estadual Campinas, Sch Chem Engn, DPQ, BR-13083970 Campinas, SP, Brazil
[3] Natl Univ San Martin, Sch Agroind Engn, Tarapoto, Peru
来源：
BIOTECHNOLOGY LETTERS | 2001年 / 23卷 / 10期
基金：
巴西圣保罗研究基金会;
关键词：
hydrophobic interaction chromatography; lipase; modelling; simulation;
D O I：
10.1023/A:1010302416897
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The Langmuir model fitted well the adsorption isotherms of lipase on the hydrophobic resin. The model parameters, Q(m) and k(d), were affected by NaCl concentration: Q(m) increased from 31 to 80 U g(-1) resin, and k(d) changed from 9.4 to 3 U ml(-1). Column modelling and the simulation data were compared with the experimental data with good agreement. The highest achieved column efficiency was 71%.
引用
收藏
页码：781 / 786
页数：6
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