Engineering genetically encoded fluorescent indicators for imaging of neuronal activity: Progress and prospects

被引：49

作者：

Shen, Yi ^{[1
]}

Nasu, Yusuke ^{[2
]}

Shkolnikov, Irene ^{[1
]}

Kim, Anna ^{[1
]}

Campbell, Robert E. ^{[1
,2
]}

机构：

[1] Univ Alberta, Dept Chem, Edmonton, AB, Canada

[2] Univ Tokyo, Grad Sch Sci, Dept Chem, Tokyo, Japan

来源：

NEUROSCIENCE RESEARCH | 2020年 / 152卷

基金：

美国国家卫生研究院; 日本学术振兴会; 加拿大自然科学与工程研究理事会;

关键词：

Genetically encoded indicators; Fluorescence imaging; Neuronal activity; Calcium ion; Membrane voltage; Neurotransmitters; NEURAL ACTIVITY; OPTICAL ELECTROPHYSIOLOGY; VOLTAGE INDICATOR; ACTION-POTENTIALS; PROTEIN; CA2+; PROBE; CALMODULIN; SENSOR; FLIES;

D O I：

10.1016/j.neures.2020.01.011

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Genetically encoded fluorescent indicators have transformed the way neuroscientists record neuronal activities and interrogate the nervous system in vivo. In this review, we discuss recent advances and new additions to the toolkit of indicators for calcium ion entry, membrane voltage change, neurotransmitter release, and other neuronal molecular processes. We highlight new engineering approaches for indicator design and development, and identify key areas for future improvement. From molecular tool developers' perspective, we aim to provide practical information for neuroscientists to evaluate and choose the most appropriate indicators for enabling new insights into brain function. (C) 2020 Elsevier B.V. and Japan Neuroscience Society. All rights reserved.

引用

页码：3 / 14

页数：12

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