Analyses of the cis-Regulatory Regions Responsible for the Transcriptional Activation of the N Resistance Gene by Tobacco mosaic virus

被引：10
|
作者：
Kobayashi, Michie [1 ]
Ishihama, Nobuaki [2 ]
Yoshioka, Hirofumi [2 ]
Takabatake, Reona [3 ]
Tsuda, Shinya [4 ]
Seo, Shigemi [1 ]
Ohashi, Yuko [1 ]
Mitsuhara, Ichiro [1 ]
机构：
[1] Natl Inst Agrobiol Sci, Plant Microbe Interact Res Unit, Tsukuba, Ibaraki 3058602, Japan
[2] Nagoya Univ, Grad Sch Bioagr Sci, Nagoya, Aichi 4648601, Japan
[3] Natl Agr & Food Res Org, Natl Food Res Inst, GMO Analyt Evaluat Lab, Tsukuba, Ibaraki 3058642, Japan
[4] Natl Agr Res Ctr, Tsukuba, Ibaraki 3058666, Japan
来源：
JOURNAL OF PHYTOPATHOLOGY | 2010年 / 158卷 / 11-12期
关键词：
N resistance gene; Tobacco mosaic virus; transcription; EXPRESSION; VECTOR;
D O I：
10.1111/j.1439-0434.2010.01686.x
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Although the tobacco N gene, which confers resistance to Tobacco mosaic virus (TMV), is transcriptionally induced by infection with TMV, the regulatory mechanism has not previously been elucidated. We found that a 5'-flanking region of the N gene exhibits TMV-inducible promoter activity and that the region from -290 to -271 includes a cis-acting element in response to infection. The activity required N protein and signal components of N-mediated resistance. These results suggest that transcription of the N gene is positively regulated by N-mediated resistance signalling.
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页码：826 / 828
页数：3
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