Assessment of bathing water quality with anE. coliimmunosensor

被引：7

作者：

Jogi, Eerik ^{[1
]}

Valing, Ingrid ^{[1
]}

Rinken, Toonika ^{[1
]}

机构：

[1] Univ Tartu, Inst Chem, Tartu, Estonia

来源：

INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY | 2022年 / 102卷 / 16期

关键词：

Escherichia coli; immunobiosensor; biological safety; bathing water; ESCHERICHIA-COLI; RAPID DETECTION; STAPHYLOCOCCUS-AUREUS; MEMBRANE; PCR; PROTEINS; SURVIVAL; BACTERIA; SAMPLES; COIL;

D O I：

10.1080/03067319.2020.1786549

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Escherichia coli is a Gram-negative, facultative anaerobic, rod-shaped coliform bacterium which is widely used as an indicator of biological safety of water. Currently, 'gold' standard for the detection ofE. coli(and other pathogens) are microbiological culture-based tests. Microbiological tests are very robust for the detection of live pathogens however it can take several days to get the results. A prospective option for the detection ofE. coliin recreational waters in addition to the genetic PCR analyses can be an immunobiosensor, allowing to detectE. coliin 20 min and to issue timely warnings of the safety of water. To compare different methods - microbiological culturing, quantitative PCR analysis and antibody-based immunobiosensor for the analysis of bathing water samples from a popular urban beach Anne Canal in Tartu, Estonia were used. The median value of biosensor results forE. coliwas considerably higher than the results of microbiological cultivation and qPCR: for 40 times, and 4 times, respectively. In addition, the biosensor results were in significant correlation with the number of total coliforms. Considering the current requirements for the biological safety of bathing water, we propose that the indicative safety threshold for bathing water usingimmunosensor analyses is in the range 4 x 10(3)-4 x 10(4)E. colicells/100 mL.

引用

页码：4652 / 4663

页数：12

共 50 条

[21] Assessment of River Water Quality During a Religious Mass Bathing Festival at Sangam, Prayagraj
LALL, A. N. U. R. A. G. S. A. M. S. O. N.
CHATTREE, A. M. I. T.
THOMAS, T. A. R. E. N. C. E.
KHAN, S. A. I. M. A. H. A. B. I. B.
PANDEY, A. V. I. N. A. S. H. K. U. M. A. R.
MANI, J. Y. O. T. I. V. A. N. D. A. N. A.
ORIENTAL JOURNAL OF CHEMISTRY, 2022, 38 (02) : 352 - 360
[22] Does the bathing water classification depend on sampling strategy? A bootstrap approach for bathing water quality assessment, according to Directive 2006/7/EC requirements
Lopez, Lago
Alvarez, Cesar
Gil, Jose L.
Revilla, Jose A.
JOURNAL OF ENVIRONMENTAL MANAGEMENT, 2012, 111 : 236 - 242
[23] CRITICAL EXAMINATION OF BATHING WATER QUALITY STANDARDS
FOSTER, DH
HANES, NB
LORD, SM
JOURNAL WATER POLLUTION CONTROL FEDERATION, 1971, 43 (11): : 2229 - &
[24] Quality of inland bathing water in Europe improves
不详
MANAGEMENT OF ENVIRONMENTAL QUALITY, 2007, 18 (06)
[25] STATISTICAL CONTROL OF HYGIENIC QUALITY OF BATHING WATER
SORENSEN, PS
JANSEN, JL
SPLIID, H
ENVIRONMENTAL MONITORING AND ASSESSMENT, 1991, 17 (2-3) : 217 - 226
[26] Weather radar to predict bathing water quality
Dale, Murray
Stidson, Ruth
WEATHER RADAR AND HYDROLOGY, 2012, 351 : 661 - +
[27] European bathing water quality improved in 2008
不详
MANAGEMENT OF ENVIRONMENTAL QUALITY, 2009, 20 (06)
[28] The effect of the time of sampling on the compliance of bathing water in NW England with the EU Directive on bathing water quality
Kwasi O.-D.
Jones K.
Paul N.
Journal of Coastal Conservation, 1999, 5 (1) : 51 - 58
[29] Bathing water signage and predictive water quality models in Scotland
McPhail, Calum D.
Stidson, Ruth T.
AQUATIC ECOSYSTEM HEALTH & MANAGEMENT, 2009, 12 (02) : 183 - 186
[30] DEBATE ON UNCERTAINTY IN ESTIMATING BATHING WATER-QUALITY
LARSEN, T
WATER SCIENCE AND TECHNOLOGY, 1992, 25 (09) : 197 - 202

← 1 2 3 4 5 →