Hoogsteen base-pairing in DNA replication? Reply

被引：3
|
作者：
Aggarwal, A [1 ]
Nair, D
Johnson, R
Prakash, L
Prakash, S
机构：
[1] Mt Sinai Sch Med, Dept Physiol & Biophys, New York, NY 10029 USA
[2] Univ Texas, Med Branch, Sealy Ctr Mol Sci, Galveston, TX 77755 USA
来源：
NATURE | 2005年 / 437卷 / 7057期
关键词：
D O I：
10.1038/nature04200
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
We have solved the crystal structure of human DNA polymerase-? (hPol?) bound to a template primer and incoming nucleotide, and shown that hPol? uses Hoogsteen base-pairing for normal DNA synthesis. Jimin Wang questions the space group we used and claims that Hoogsteen base-pairing is not supported by our electron-density maps. We show here that the issue of space group does not affect our conclusions and that our electron-density maps are clear in indicating that Hoogsteen and not Watson-Crick base-pairing occurs in this situation. © 2005 by Nature Publishing Group.
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收藏
页码：E7 / E7
页数：1
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