Establishment of PLAFMCi004-A induced pluripotent stem cells derived from PBMCs from a healthy individual

被引：0
|
作者：
Shang, Shunlai [1 ,2 ]
Chen, Keng [3 ]
Cai, Guangyan [2 ]
Bai, Xueyuan [2 ]
Chen, Xiangmei [1 ,2 ]
Li, Qinggang [2 ]
机构：
[1] Nankai Univ, Sch Med, Tianjin 300071, Peoples R China
[2] Chinese Peoples Liberat Army Gen Hosp, Chinese PLA Inst Nephrol, Med Sch Chinese PLA,Med Ctr 1,Dept Nephrol, State Key Lab Kidney Dis,Natl Clin Res Ctr Kidney, Beijing 100853, Peoples R China
[3] Guangdong Pharmaceut Univ, Sch Clin Med, Guangzhou, Peoples R China
来源：
STEM CELL RESEARCH | 2021年 / 53卷
基金：
北京市自然科学基金; 中国国家自然科学基金;
关键词：
D O I：
10.1016/j.scr.2021.102316
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
Peripheral blood mononuclear cells (PBMCs) were isolated from a healthy male individual. PBMCs were reprogrammed into human induced pluripotent stem cells (iPSCs) using plasmids carrying the following reprogramming factors: NANOG, LIN28, OCT4, SOX2, c-MYC, and KLF4. This cell line expressed pluripotency markers, exhibited the normal male karyotype (46, XY), and had the potential to differentiate into 3 germ layers, as confirmed by techniques such as flow cytometry. The iPSC obtained from a healthy individual can be used for organoids to reveal developmental mechanism of tissue and organ regeneration, stem cell therapy and drug screening.
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页数：4
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