Establishment of PLAFMCi004-A induced pluripotent stem cells derived from PBMCs from a healthy individual

被引：0

作者：

Shang, Shunlai ^{[1
,2
]}

Chen, Keng ^{[3
]}

Cai, Guangyan ^{[2
]}

Bai, Xueyuan ^{[2
]}

Chen, Xiangmei ^{[1
,2
]}

Li, Qinggang ^{[2
]}

机构：

[1] Nankai Univ, Sch Med, Tianjin 300071, Peoples R China

[2] Chinese Peoples Liberat Army Gen Hosp, Chinese PLA Inst Nephrol, Med Sch Chinese PLA,Med Ctr 1,Dept Nephrol, State Key Lab Kidney Dis,Natl Clin Res Ctr Kidney, Beijing 100853, Peoples R China

[3] Guangdong Pharmaceut Univ, Sch Clin Med, Guangzhou, Peoples R China

来源：

STEM CELL RESEARCH | 2021年 / 53卷

基金：

北京市自然科学基金; 中国国家自然科学基金;

关键词：

D O I：

10.1016/j.scr.2021.102316

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Peripheral blood mononuclear cells (PBMCs) were isolated from a healthy male individual. PBMCs were reprogrammed into human induced pluripotent stem cells (iPSCs) using plasmids carrying the following reprogramming factors: NANOG, LIN28, OCT4, SOX2, c-MYC, and KLF4. This cell line expressed pluripotency markers, exhibited the normal male karyotype (46, XY), and had the potential to differentiate into 3 germ layers, as confirmed by techniques such as flow cytometry. The iPSC obtained from a healthy individual can be used for organoids to reveal developmental mechanism of tissue and organ regeneration, stem cell therapy and drug screening.

引用

页数：4

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