The Great Escape: mRNA Export through the Nuclear Pore Complex

被引:21
|
作者
De Magistris, Paola [1 ]
机构
[1] Delft Univ Technol, Kavli Inst Nanosci, Bionanosci Dept, NL-2629 HZ Delft, Netherlands
关键词
mRNA; mRNP; NPC; nuclear transport; nuclear export; Mex67/MTR2; NXF1/NXT1; Sub2/UAP56; Yra1/Aly; BOX PROTEIN DBP5; STRUCTURAL BASIS; BINDING PROTEIN; FG-NUCLEOPORINS; NUCLEOCYTOPLASMIC TRANSPORT; SACCHAROMYCES-CEREVISIAE; CONSERVED INTERACTION; KINETIC-ANALYSIS; TERMINAL DOMAIN; POLY(A)(+) RNA;
D O I
10.3390/ijms222111767
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Nuclear export of messenger RNA (mRNA) through the nuclear pore complex (NPC) is an indispensable step to ensure protein translation in the cytoplasm of eukaryotic cells. mRNA is not translocated on its own, but it forms ribonuclear particles (mRNPs) in association with proteins that are crucial for its metabolism, some of which; like Mex67/MTR2-NXF1/NXT1; are key players for its translocation to the cytoplasm. In this review, I will summarize our current body of knowledge on the basic characteristics of mRNA export through the NPC. To be granted passage, the mRNP cargo needs to bind transport receptors, which facilitate the nuclear export. During NPC transport, mRNPs undergo compositional and conformational changes. The interactions between mRNP and the central channel of NPC are described; together with the multiple quality control steps that mRNPs undergo at the different rings of the NPC to ensure only proper export of mature transcripts to the cytoplasm. I conclude by mentioning new opportunities that arise from bottom up approaches for a mechanistic understanding of nuclear export.
引用
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页数:20
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