Enhancement of phenyllactic acid biosynthesis by recognition site replacement of D-lactate dehydrogenase from Lactobacillus pentosus

被引：25
|
作者：
Zhu, Yibo [1 ,2 ]
Hu, Fagen [1 ,2 ,3 ]
Zhu, Yingyue [1 ,2 ]
Wang, Limei [1 ,2 ]
Qi, Bin [1 ,2 ]
机构：
[1] Changshu Inst Technol, Sch Biotechnol & Food Engn, Changshu 215500, Jiangsu, Peoples R China
[2] Changshu Inst Technol, Key Lab Food & Biotechnol Suzhou, Changshu 215500, Jiangsu, Peoples R China
[3] Soochow Univ, Sch Biol & Basic Med Sci, Suzhou 215123, Jiangsu, Peoples R China
来源：
BIOTECHNOLOGY LETTERS | 2015年 / 37卷 / 06期
基金：
中国国家自然科学基金;
关键词：
D-Lactate dehydrogenase; 3-Phenyllactic acid; Phenylpyruvic acid; Recognition site residue replacement; Whole-cell transformation; PHENYLPYRUVIC ACID; BIOTRANSFORMATION; CONVERSION; SUBSTRATE; SPECTRUM; FORMATE;
D O I：
10.1007/s10529-015-1778-4
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The Tyr52 residue of d-lactate dehydrogenase (d-LDH) from Lactobacillus pentosus was replaced with small hydrophobic residues and overexpressed in E. coli BL21 (DE3) to enhance 3-phenyllactic acid (PLA) synthesis by whole-cell catalyst. Escherichia coli pET-28a-d-ldh produced 12.2 g PLA l(-1) in 3 h, with a molar conversion rate of 61 %, while E. coli pET-28a-d-ldh (Y52V) produced 15.6 g PLA l(-1), with a molar conversion rate of 77 %. This study demonstrates the feasibility of using engineered E. coli for PLA production from phenylpyruvate (PPA) and showed that site-directed mutagenesis of d-ldh markedly improved PLA yield and substrate conversion rate. This biocatalytic system is a promising platform for PLA production from PPA.
引用
收藏
页码：1233 / 1241
页数：9
相关论文
共 50 条
  • [21] Discovery and characterization of a thermostable D-lactate dehydrogenase from Lactobacillus jensenii through genome mining
    Jun, Chanha
    Sa, Young Seung
    Gu, Sol-A
    Joo, Jeong Chan
    Kim, Seil
    Kim, Kyung-Jin
    Kim, Yong Hwan
    PROCESS BIOCHEMISTRY, 2013, 48 (01) : 109 - 117
  • [22] Classification and identification of strains of Lactobacillus brevis based on electrophoretic characterization of D-Lactate dehydrogenase:: Relationship between D-Lactate dehydrogenase and beer-spoilage ability
    Takahashi, T
    Nakakita, Y
    Sugiyama, H
    Shigyo, T
    Shinotsuka, K
    JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 1999, 88 (05) : 500 - 506
  • [23] Enhancement of lactate fraction in poly(lactate-co-3-hydroxybutyrate) synthesized by Escherichia coli harboring the D-lactate dehydrogenase gene from Lactobacillus acetotolerans HT
    Goto, Saki
    Suzuki, Naoyuki
    Matsumoto, Ken'ichiro
    Taguchi, Seiichi
    Tanaka, Kenji
    Matsusaki, Hiromi
    JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY, 2019, 65 (04): : 204 - 208
  • [24] Enantioselective Biosynthesis of L-Phenyllactic Acid From Phenylpyruvic Acid In Vitro by L-Lactate Dehydrogenase Coupling With Glucose Dehydrogenase
    Zhang, Dong
    Zhang, Ting
    Lei, Yuqing
    Lin, Wenqian
    Chen, Xingyi
    Wu, Minchen
    FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, 2022, 10
  • [25] D-LACTATE DEHYDROGENASE FROM HORSESHOE CRAB - BIOL
    LONG, GL
    KAPLAN, NO
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 1970, (SEP): : 103 - &
  • [26] Cloning of D-lactate dehydrogenase genes of Lactobacillus delbrueckii subsp bulgaricus and their roles in D-lactic acid production
    Huang, Yanna
    You, Chunping
    Liu, Zhenmin
    3 BIOTECH, 2017, 7
  • [27] A single point mutation engineering for changing the substrate specificity of D-lactate dehydrogenase from Lactobacillus fermentum
    Fan, Xinyu
    Bai, Yajun
    Fan, Tai-Ping
    Zheng, Xiaohui
    Cai, Yujie
    LWT-FOOD SCIENCE AND TECHNOLOGY, 2021, 151
  • [28] Lactate racemization as a rescue pathway for supplying D-lactate to the cell wall biosynthesis machinery in Lactobacillus plantarum
    Goffin, P
    Deghorain, M
    Mainardi, JL
    Tytgat, I
    Champomier-Vergès, MC
    Kleerebezem, M
    Hols, P
    JOURNAL OF BACTERIOLOGY, 2005, 187 (19) : 6750 - 6761
  • [29] HISTIDINE-296 IS ESSENTIAL FOR THE CATALYSIS IN LACTOBACILLUS-PLANTARUM D-LACTATE DEHYDROGENASE
    TAGUCHI, H
    OHTA, T
    JOURNAL OF BIOLOGICAL CHEMISTRY, 1993, 268 (24) : 18030 - 18034
  • [30] Cloning of d-lactate dehydrogenase genes of Lactobacillus delbrueckii subsp. bulgaricus and their roles in d-lactic acid production
    Yanna Huang
    Chunping You
    Zhenmin Liu
    3 Biotech, 2017, 7
12345