Enhancement of phenyllactic acid biosynthesis by recognition site replacement of D-lactate dehydrogenase from Lactobacillus pentosus

被引：25
|
作者：
Zhu, Yibo [1 ,2 ]
Hu, Fagen [1 ,2 ,3 ]
Zhu, Yingyue [1 ,2 ]
Wang, Limei [1 ,2 ]
Qi, Bin [1 ,2 ]
机构：
[1] Changshu Inst Technol, Sch Biotechnol & Food Engn, Changshu 215500, Jiangsu, Peoples R China
[2] Changshu Inst Technol, Key Lab Food & Biotechnol Suzhou, Changshu 215500, Jiangsu, Peoples R China
[3] Soochow Univ, Sch Biol & Basic Med Sci, Suzhou 215123, Jiangsu, Peoples R China
来源：
BIOTECHNOLOGY LETTERS | 2015年 / 37卷 / 06期
基金：
中国国家自然科学基金;
关键词：
D-Lactate dehydrogenase; 3-Phenyllactic acid; Phenylpyruvic acid; Recognition site residue replacement; Whole-cell transformation; PHENYLPYRUVIC ACID; BIOTRANSFORMATION; CONVERSION; SUBSTRATE; SPECTRUM; FORMATE;
D O I：
10.1007/s10529-015-1778-4
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The Tyr52 residue of d-lactate dehydrogenase (d-LDH) from Lactobacillus pentosus was replaced with small hydrophobic residues and overexpressed in E. coli BL21 (DE3) to enhance 3-phenyllactic acid (PLA) synthesis by whole-cell catalyst. Escherichia coli pET-28a-d-ldh produced 12.2 g PLA l(-1) in 3 h, with a molar conversion rate of 61 %, while E. coli pET-28a-d-ldh (Y52V) produced 15.6 g PLA l(-1), with a molar conversion rate of 77 %. This study demonstrates the feasibility of using engineered E. coli for PLA production from phenylpyruvate (PPA) and showed that site-directed mutagenesis of d-ldh markedly improved PLA yield and substrate conversion rate. This biocatalytic system is a promising platform for PLA production from PPA.
引用
收藏
页码：1233 / 1241
页数：9
相关论文
共 50 条
  • [1] Enhancement of phenyllactic acid biosynthesis by recognition site replacement of D-lactate dehydrogenase from Lactobacillus pentosus
    Yibo Zhu
    Fagen Hu
    Yingyue Zhu
    Limei Wang
    Bin Qi
    Biotechnology Letters, 2015, 37 : 1233 - 1241
  • [2] Conversion of Lactobacillus pentosus D-lactate dehydrogenase to a D-hydroxyisocaproate dehydrogenase through a single amino acid replacement
    Tokuda, C
    Ishikura, Y
    Shigematsu, M
    Mutoh, H
    Tsuzuki, S
    Nakahira, Y
    Tamura, Y
    Shinoda, T
    Arai, K
    Takahashi, O
    Taguchi, H
    JOURNAL OF BACTERIOLOGY, 2003, 185 (16) : 5023 - 5026
  • [3] Enzymological characterization of a novel d-lactate dehydrogenase from Lactobacillus rossiae and its application in d-phenyllactic acid synthesis
    Xi Luo
    Yingying Zhang
    Fengwei Yin
    Gaowei Hu
    Qiang Jia
    Changsheng Yao
    Yongqian Fu
    3 Biotech, 2020, 10
  • [4] Enzymological characterization of a novel d-lactate dehydrogenase from Lactobacillus rossiae and its application in d-phenyllactic acid synthesis
    Luo, Xi
    Zhang, Yingying
    Yin, Fengwei
    Hu, Gaowei
    Jia, Qiang
    Yao, Changsheng
    Fu, Yongqian
    3 BIOTECH, 2020, 10 (03)
  • [5] Characterization of D-lactate dehydrogenase from Pediococcus acidilactici that converts phenylpyruvic acid into phenyllactic acid
    Mu, Wanmeng
    Yu, Shuhuai
    Jiang, Bo
    Li, Xingfeng
    BIOTECHNOLOGY LETTERS, 2012, 34 (05) : 907 - 911
  • [6] Characterization of d-lactate dehydrogenase from Pediococcus acidilactici that converts phenylpyruvic acid into phenyllactic acid
    Wanmeng Mu
    Shuhuai Yu
    Bo Jiang
    Xingfeng Li
    Biotechnology Letters, 2012, 34 : 907 - 911
  • [7] Characterization of D-Lactate Dehydrogenase Producing D-3-Phenyllactic Acid from Pediococcus pentosaceus
    Yu, Shuhuai
    Jiang, Houyi
    Jiang, Bo
    Mu, Wanmeng
    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2012, 76 (04) : 853 - 855
  • [8] Insights into substrate binding by D-2-ketoacid dehydrogenases from the structure of Lactobacillus pentosus D-lactate dehydrogenase
    Stoll, VS
    Kimber, MS
    Pai, EF
    STRUCTURE, 1996, 4 (04) : 437 - 447
  • [9] CRYSTALLIZATION OF D-LACTATE DEHYDROGENASE FROM LACTOBACILLUS-BULGARICUS
    NESSLER, S
    LEBRAS, G
    LEBRAS, G
    GAREL, JR
    JOURNAL OF MOLECULAR BIOLOGY, 1994, 235 (01) : 370 - 371
  • [10] Efficient synthesis of D-phenyllactic acid by a whole-cell biocatalyst co-expressing glucose dehydrogenase and a novel D-lactate dehydrogenase from Lactobacillus rossiae
    Luo, Xi
    Zhang, Yingying
    Yin, Longfei
    Zheng, Weilong
    Fu, Yongqian
    3 BIOTECH, 2020, 10 (01)
12345