Rapid and Sensitive Detection of Bovine Coronavirus and Group A Bovine Rotavirus from Fecal Samples by Using One-Step Duplex RT-PCR Assay

被引：15

作者：

Zhu, Wei ^{[1
]}

Dong, Jianbao ^{[1
]}

Haga, Takeshi ^{[1
]}

Goto, Yoshitaka ^{[1
]}

Sueyoshi, Masuo ^{[2
]}

机构：

[1] Miyazaki Univ, Dept Vet Microbiol, Miyazaki 8892192, Japan

[2] Miyazaki Univ, Dept Vet Hyg, Miyazaki 8892192, Japan

来源：

JOURNAL OF VETERINARY MEDICAL SCIENCE | 2011年 / 73卷 / 04期

关键词：

BCoV; detection; differentiation; group A BRV; one-step duplex RT-PCR; WINTER DYSENTERY; CALF DIARRHEA; NESTED PCR; VIRUS; DIAGNOSIS; ENTERO;

D O I：

10.1292/jvms.10-0401

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Bovine coronavirus (BCoV) and group A bovine rotavirus (BRV) are two of major causes for neonatal calf diarrhea. In the present study, a one-step duplex RT-PCR was established to detect and differentiate BCoV and group A BRV from fecal samples. The sensitivity of this method for BCoV and group A BRV was 10 PFU/100 mu l and I PFU/100 mu l, respectively. Twenty-eight diarrhea fecal samples were detected with this method, the result showed that 2 samples were identified as co-infected with BCoV and group A BRV, 26 samples were group A BRV positive, and 2 samples were negative. It proved that this method is sensitive for clinical fecal samples and is worth applying to laboratory diagnosis for BCoV and group A BRV.

引用

页码：531 / 534

页数：4

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