Rapid and Sensitive Detection of Bovine Coronavirus and Group A Bovine Rotavirus from Fecal Samples by Using One-Step Duplex RT-PCR Assay

被引：15

作者：

Zhu, Wei ^{[1
]}

Dong, Jianbao ^{[1
]}

Haga, Takeshi ^{[1
]}

Goto, Yoshitaka ^{[1
]}

Sueyoshi, Masuo ^{[2
]}

机构：

[1] Miyazaki Univ, Dept Vet Microbiol, Miyazaki 8892192, Japan

[2] Miyazaki Univ, Dept Vet Hyg, Miyazaki 8892192, Japan

来源：

JOURNAL OF VETERINARY MEDICAL SCIENCE | 2011年 / 73卷 / 04期

关键词：

BCoV; detection; differentiation; group A BRV; one-step duplex RT-PCR; WINTER DYSENTERY; CALF DIARRHEA; NESTED PCR; VIRUS; DIAGNOSIS; ENTERO;

D O I：

10.1292/jvms.10-0401

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Bovine coronavirus (BCoV) and group A bovine rotavirus (BRV) are two of major causes for neonatal calf diarrhea. In the present study, a one-step duplex RT-PCR was established to detect and differentiate BCoV and group A BRV from fecal samples. The sensitivity of this method for BCoV and group A BRV was 10 PFU/100 mu l and I PFU/100 mu l, respectively. Twenty-eight diarrhea fecal samples were detected with this method, the result showed that 2 samples were identified as co-infected with BCoV and group A BRV, 26 samples were group A BRV positive, and 2 samples were negative. It proved that this method is sensitive for clinical fecal samples and is worth applying to laboratory diagnosis for BCoV and group A BRV.

引用

页码：531 / 534

页数：4

共 50 条

[1] Rapid Detection of Sacbrood Virus (SBV) by One-Step RT-PCR Assay
Shen, Ke-Fei
Lan-Cao
Yang, Jin-Long
JOURNAL OF ANIMAL AND VETERINARY ADVANCES, 2012, 11 (07): : 931 - 932
[2] Rapid detection of bovine coronavirus by a semi-nested RT-PCR
Asano, Karen M.
Souza, Sibele P.
Silva, Sheila O. S.
Richtzenhain, Leonardo J.
Brandao, Paulo E.
PESQUISA VETERINARIA BRASILEIRA, 2009, 29 (11): : 869 - 873
[3] One-step quantitative RT-PCR for the detection of rotavirus in acute gastroenteritis
Zeng, S. -Q.
Halkosalo, A.
Salminen, M.
Szakal, E. D.
Puustinen, L.
Vesikari, T.
JOURNAL OF VIROLOGICAL METHODS, 2008, 153 (02) : 238 - 240
[4] Detection of Bovine Group A Rotavirus Using Rapid Antigen Detection Kits, RT-PCR and Next-Generation DNA Sequencing
Minami-Fukuda, Fujiko
Nagai, Makoto
Takai, Hikaru
Murakami, Toshiaki
Ozawa, Tadashi
Tsuchiaka, Shinobu
Okazaki, Sachiko
Katayama, Yukie
Oba, Mami
Nishiura, Naomi
Sassa, Yukiko
Omatsu, Tsutomu
Furuya, Tetsuya
Koyama, Satoshi
Shirai, Junsuke
Tsunemitsu, Hiroshi
Fujii, Yoshiki
Katayama, Kazuhiko
Mizutani, Tetsuya
JOURNAL OF VETERINARY MEDICAL SCIENCE, 2013, 75 (12): : 1651 - 1655
[5] Rapid detection of Enterovirus and Coxsackievirus A10 by a TaqMan based duplex one-step real time RT-PCR assay
Chen, Jingfang
Zhang, Rusheng
Ou, Xinhua
Yao, Dong
Huang, Zheng
Li, Linzhi
Sun, Biancheng
MOLECULAR AND CELLULAR PROBES, 2017, 33 : 8 - 10
[6] Development of a one-step RT-PCR assay for detection of pancoronaviruses (α-, β-, γ- and δ-coronaviruses) using newly designed degenerate primers for porcine and avian 'fecal samples
Hu, Hui
Jung, Kwonil
Wang, Qiuhong
Saif, Linda J.
Vlasova, Anastasia N.
JOURNAL OF VIROLOGICAL METHODS, 2018, 256 : 116 - 122
[7] Rapid detection of infectious bursal disease virus using one-step RT-PCR in clinical samples in Pakistan
Zahoor, M. A.
Hussain, I.
Mansoor, M. K.
Masood, S.
Khan, Q. M.
POULTRY SCIENCE, 2004, 83 : 109 - 109
[8] Rapid detection of infectious bursal disease virus using one-step RT-PCR in clinical samples in Pakistan
Zahoor, M. A.
Hussain, I.
Mansoor, M. K.
Masood, S.
Khan, Q. M.
JOURNAL OF DAIRY SCIENCE, 2004, 87 : 109 - 109
[9] Rapid detection of infectious bursal disease virus using one-step RT-PCR in clinical samples in Pakistan
Zahoor, M. A.
Hussain, I.
Mansoor, M. K.
Masood, S.
Khan, Q. M.
JOURNAL OF ANIMAL SCIENCE, 2004, 82 : 109 - 109
[10] Quantitative one-step RT-PCR assay for rapid and sensitive identification and titration of polioviruses in clinical specimens
Laassri, Majid
DiPiazza, Anthony
Bidzhieva, Bella
Zagorodnyaya, Tatiana
Chumakov, Konstantin
JOURNAL OF VIROLOGICAL METHODS, 2013, 189 (01) : 7 - 14

← 1 2 3 4 5 →