Real-time fluorescence assays of alkaline phosphatase and ATP sulfurylase activities based on a novel PPi fluorescent probe

被引：27
|
作者：
Wang, Xiaobo [1 ]
Zhang, Zhiyang [1 ]
Ma, Xiaoyan [1 ]
Wen, Jinghan [1 ]
Geng, Zhirong [1 ]
Wang, Zhilin [1 ]
机构：
[1] Nanjing Univ, Sch Chem & Chem Engn, Collaborat Innovat Ctr Adv Microstruct, State Key Lab Coordinat Chem, Nanjing 210093, Jiangsu, Peoples R China
来源：
TALANTA | 2015年 / 137卷
基金：
中国国家自然科学基金;
关键词：
PPi; Fluorescent probe; Alkaline phosphatase; ATP sulfurylase; Real-time fluorescence assay; ADENOSINE 5'-PHOSPHOSULFATE KINASE; RHEUMATOID-ARTHRITIS; INORGANIC PYROPHOSPHATE; MECHANISM; ARABIDOPSIS; HYDROLYSIS; METABOLISM; BIOSENSOR; DISEASE; STEP;
D O I：
10.1016/j.talanta.2015.01.028
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
An anthracene-armed tetraaza macrocyclic fluorescent probe 3-(9-anthrylmethyl)-3,6,9,15-tetraazabicyclo[9.3.1]pentadeca-1(15),11,13-triene(L) for detecting Zn2+ in aqueous medium was synthesized. L-Zn2+ complex, showed selectivity toward pyrophosphate ion (PPi) by quenching the fluorescence in aqueous HEPES buffer (pH 7.4). Furthermore, L-Zn2+ was also used to set up a real-time fluorescence assay for monitoring enzyme activities of alkaline phosphatase (ALP) and adenosine triphosphate sulfurylase (ATPS). In the presence of ALP inhibitor Na3VO4 and ATPS inhibitor chlorate, two enzymes activities decreased obviously, respectively. (C) 2015 Elsevier B.V. All rights reserved.
引用
收藏
页码：156 / 160
页数：5
相关论文
共 50 条
  • [41] Real-time monitoring of abnormal mitochondrial viscosity in glioblastoma with a novel mitochondria-targeting fluorescent probe
    Zhang, Qing
    Liu, Wei
    Jiang, Ling
    He, Yan-Jun
    Wu, Chang-Jian
    Ren, Shen-Zhen
    Wang, Bao-Zhong
    Liu, Li
    Zhu, Hai-Liang
    Wang, Zhong-Chang
    ANALYST, 2024, 149 (10) : 2956 - 2965
  • [42] Real-time intelligent detection of ethephon based on a high-throughput ratiometric fluorescent probe
    Chang, Hongrong
    Tan, Ping
    Chen, Xianjin
    Liu, Tao
    Lu, Zhiwei
    Sun, Mengmeng
    Su, Gehong
    Wang, Yanying
    Zou, Yuanfeng
    Rao, Hanbing
    Wu, Chun
    JOURNAL OF HAZARDOUS MATERIALS, 2024, 465
  • [43] An Activity-Based Ratiometric Fluorescent Probe for In Vivo Real-Time Imaging of Hydrogen Molecules
    Gong, Wanjun
    Jiang, Lingdong
    Zhu, Yanxia
    Jiang, Mengna
    Chen, Danyang
    Jin, Zhaokui
    Qin, Shucun
    Yu, Zhiqiang
    He, Qianjun
    ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2022, 61 (09)
  • [44] Real-Time Monitoring of Self-Aggregation of β-Amyloid by a Fluorescent Probe Based on Ruthenium Complex
    Yu, Hui-juan
    Zhao, Wei
    Xie, Mengting
    Li, Xiaoqing
    Sun, Ming
    He, Jun
    Wang, Lu
    Yu, Lin
    ANALYTICAL CHEMISTRY, 2020, 92 (04) : 2953 - 2960
  • [45] Reversible Reaction-Based Fluorescent Probe for Real-Time Imaging of Glutathione Dynamics in Mitochondria
    Chen, Jianwei
    Jiang, Xiqian
    Zhang, Chengwei
    MacKenzie, Kevin R.
    Stossi, Fabio
    Palzkill, Timothy
    Wang, Meng C.
    Wang, Jin
    ACS SENSORS, 2017, 2 (09): : 1257 - 1261
  • [46] Real-time fluorescence assay of alkaline phosphatase in living cells using boron-doped graphene quantum dots as fluorophores
    Chen, Li
    Yang, Guancao
    Wu, Ping
    Cai, Chenxin
    BIOSENSORS & BIOELECTRONICS, 2017, 96 : 294 - 299
  • [47] Fluorescence resonance energy transfer-based assays for the real-time detection of nitric oxide signaling
    St Croix, CM
    Stitt, MS
    Watkins, SC
    Pitt, BR
    NITRIC OXIDE, PT E, 2005, 396 : 317 - +
  • [48] Two birds with one stone: A novel dual-functional fluorescent probe for simultaneous monitoring and real-time imaging of alkaline pH and viscosity in living cells
    Zhang, Yan
    Qin, Ahui
    Gong, Shuai
    Li, Mingxin
    Meng, Zhiyuan
    Liang, Yueyin
    Shen, Zheyu
    Wang, Zhonglong
    Wang, Shifa
    MICROCHEMICAL JOURNAL, 2022, 173
  • [49] Real-time label-free quantitative fluorescence microscopy-based detection of ATP using a tunable fluorescent nano-aptasensor platform
    Shrivastava, Sajal
    Sohn, Il-Yung
    Son, Young-Min
    Lee, Won-Il
    Lee, Nae-Eung
    NANOSCALE, 2015, 7 (46) : 19663 - 19672
  • [50] Companion Diagnosis for Retinal Neuroprotective Treatment by Real-Time Imaging of Calpain Activation Using a Novel Fluorescent Probe
    Asano, Toshifumi
    Nagayo, Yuri
    Tsuda, Satoru
    Ito, Azusa
    Kobayashi, Wataru
    Fujita, Kosuke
    Sato, Kota
    Nishiguchi, Koji M.
    Kunikata, Hiroshi
    Fujioka, Hiroyoshi
    Kamiya, Mako
    Urano, Yasuteru
    Nakazawa, Toru
    BIOCONJUGATE CHEMISTRY, 2020, 31 (09) : 2241 - 2251
12345