Valorizing a hydrothermal liquefaction aqueous phase through co-production of chemicals and lipids using the oleaginous yeast Yarrowia lipolytica

被引：35

作者：

Cordova, Lauren T. ^{[1
]}

Lad, Beena C. ^{[2
]}

Ali, Sabah A. ^{[1
]}

Schmidt, Andrew J. ^{[3
]}

Billing, Justin M. ^{[3
]}

Pomraning, Kyle ^{[3
]}

Hofstad, Beth ^{[3
]}

Swita, Marie S. ^{[3
]}

Collett, James R. ^{[3
]}

Alper, Hal S. ^{[1
,4
]}

机构：

[1] Univ Texas Austin, McKetta Dept Chem Engn, 200 E Dean Keeton St Stop C0400, Austin, TX 78712 USA

[2] Univ Texas Austin, Dept Mol Biosci, 100 East 24th St Stop A5000, Austin, TX 78712 USA

[3] Pacific Northwest Natl Lab, Chem & Biol Proc Grp, Richland, WA 99352 USA

[4] Univ Texas Austin, Inst Cellular & Mol Biol, 2500 Speedway Ave, Austin, TX 78712 USA

来源：

BIORESOURCE TECHNOLOGY | 2020年 / 313卷

关键词：

Hydrothermal Liquefaction Aqueous Phase; Yarrowia lipolytica; Co-production; TRIACETIC ACID LACTONE; VOLATILE FATTY-ACIDS; ANAEROBIC-DIGESTION; WASTE-WATER; RENEWABLE DIESEL; BIOCONVERSION; ALGAE;

D O I：

10.1016/j.biortech.2020.123639

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

Hydrothermal liquefaction is a promising technology to upgrade wet organic waste into a biocrude oil for diesel or jet fuel; however, this process generates an acid-rich aqueous phase which poses disposal issues. This hydrothermal liquefaction aqueous phase (HTL-AP) contains organic acids, phenol, and other toxins. This work demonstrates that Y. lipolytica as a unique host to valorize HTL-AP into a variety of co-products. Specifically, strains of Y. lipolytica can tolerate HTL-AP at 10% in defined media and 25% in rich media. The addition of HTLAP enhances production of the polymer precursor itaconic acid by 3-fold and the polyketide triacetic acid lactone at least 2-fold. Additional co-products (lipids and citric acid) were produced in these fermentations. Finally, bioreactor cultivation enabled 21.6 g/L triacetic acid lactone from 20% HTL-AP in mixed sugar hydrolysate. These results demonstrate the first use of Y. lipolytica in HTL-AP valorization toward production of a portfolio of value-added compounds.

引用

页数：9