The retention behavior of solutes in anionic micellar liquid chromatography

The retention characteristics of solutes in anionic micellar liquid chromatography (AMLC) were investigated in terms of three-phase equilibrium theory, using an anionic surfactant (sodium dodecyl sulfate) solution as mobile phase. Acidic, basic and amphoteric solutes were investigated. The retention of solutes was depended on the assembled interaction of solutes with the micelles in the mobile phase and with the modified stationary phase. When adding organic modifier, the partitioning equilibria between aqueous phase and modified stationary phase, aqueous phase and micellar phase were significantly altered. Also, the accordingly retention behavior of solutes was managed by the balance of hydrophobic and electrostatic interaction. Moreover, there was good correlation between lipophilicity from MLC, immobilized artificial membrane (IAM) and n-octanot/buffer, and minimal inhibitory concentration of three microbes for p-hydroxybenzoic ester anologs and it suggestive that analogs their inhibitory mechanism may be associated with membrane affinity.