Replication and transcription of human papillomavirus type 58 genome in Saccharomyces cerevisiae

被引：2

作者：

Li, Jing ^{[1
]}

Wang, Xiao ^{[2
]}

Liu, Juan ^{[1
]}

Wang, Hong ^{[1
]}

Zhang, Xiao-Li ^{[1
]}

Tang, Wei ^{[1
]}

Sun, Yun-Dong ^{[1
]}

Wang, Xin ^{[1
]}

Yu, Xiu-Ping ^{[1
]}

Zhao, Wei-Ming ^{[1
]}

机构：

[1] Shandong Univ, Sch Med, Dept Med Microbiol, Jinan 250012, Shandong, Peoples R China

[2] Shandong Univ, Sch Med, Dept Pathol, Jinan 250012, Shandong, Peoples R China

来源：

VIROLOGY JOURNAL | 2010年 / 7卷

基金：

中国国家自然科学基金;

关键词：

BOVINE PAPILLOMAVIRUS; IDENTIFICATION; TRANSFORMATION; YEAST; MAINTENANCE; ELEMENTS; REGION; ORIGIN; SITES;

D O I：

10.1186/1743-422X-7-368

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Background: To establish a convenient system for the study of human papillomavirus (HPV), we inserted a Saccharomyces cerevisiae selectable marker, Ura, into HPV58 genome and transformed it into yeast. Results: HPV58 genome could replicate extrachromosomally in yeast, with transcription of its early and late genes. However, with mutation of the viral E2 gene, HPV58 genome lost its mitotic stability, and the transcription levels of E6 and E7 genes were upregulated. Conclusions: E2 protein could participate in viral genome maintenance, replication and transcription regulation. This yeast model could be used for the study of certain aspects of HPV life cycle.

引用

页数：8

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