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Two-photon fluorescence anisotropy imaging
被引：0
|作者：
Wang, XF
Wang, Y
Jiang, Y
Ma, H
[1
]
机构：
[1] Tsing Hua Univ, Dept Phys, Minist Educ, Key Lab Atom & Mol Neurosci, Beijing 100084, Peoples R China
[2] Tsing Hua Univ, Grad Sch Shenzhen, Shenzhen 518057, Peoples R China
[3] Beijing Univ Chinese Med, Dept Pathol, Beijing 100029, Peoples R China
关键词：
fluorescence anisotropy;
two-photon scanning microscopy;
fluidity of membrane;
berberine;
tumor cell;
D O I：
暂无
中图分类号：
Q5 [生物化学];
Q7 [分子生物学];
学科分类号：
071010 ;
081704 ;
摘要：
A new method is developed for two photon fluorescence anisotropy imaging. Its biological applications are tested. This system is based on a two-photon laser scanning fluorescence microscope. A polarization beam splitter was inserted into the optical path to separated fluorescence into components of orthogonal polarization. By rotating the polarization of the excitation beam by 90degrees, four images were collected for each sample. These images were then processed pixel-by-pixel to generate a new fluorescence anisotropy image. The capability of this method is tested for different samples, including FITC, FITC-CD44 in solution and FITC-CD44 attached to the membranes of tumor cells. The results show that fluorescence images can distinguish fluorescence molecules of different molecular mass, or detect changes in microenvironment.
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页码：161 / 167
页数：7
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