Isolation and nucleotide sequence of the gene encoding phosphoenolpyruvate carboxykinase from Kluyveromyces lactis

被引：0
|
作者：
Kitamoto, HK
Ohmomo, S
Iimura, Y
机构：
[1] Inst Recursos Nat & Agrobiol, Ibaraki, Osaka 305, Japan
[2] Natl Inst Anim Ind, Ibaraki, Osaka 305, Japan
[3] Yamanashi Univ, Dept Appl Chem & Biotechnol, Kofu, Yamanashi 400, Japan
来源：
YEAST | 1998年 / 14卷 / 10期
关键词：
gluconeogenesis; PEPCK; Kluyveromyces lactis;
D O I：
10.1002/(SICI)1097-0061(199807)14:10<963::AID-YEA282>3.0.CO;2-X
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The KIPCK1 gene encoding phosphoenolpyruvate carboxykinase (PEPCK; ATP-dependent) was cloned from the Kluyveromyces lactis genome using a PCR amplicon from Saccharomyces cerevisiae PCK1 gene as a probe. A DNA fragment of about 4.8 kb containing KIPCK1 complemented PEPCK activity of the mutant of S. cerevisiae defective in PEPCK. The KIPCK1 gene has an open reading frame of 1629 bp (543 amino acids). The KIPCK1 nucleotide sequence and deduced amino acid sequence showed 76% and 84% homologies to those of S. cerevisiae PCK1, respectively. Multiple alignment of ATP-dependent PEPCK genes shows highly conserved regions. The nucleotide sequence of KIPCK1 has been submitted to the DDBJ/GenBank/EMBL data bank with Accession Number U88575. (C) 1998 John Wiley & Sons, Ltd.
引用
收藏
页码：963 / 967
页数：5
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