Reprogramming of mouse neural stem cells to induced pluripotent stem cells using Oct4 combined with microRNA

被引：1
|
作者：
Yan, Qiuyue [1 ]
Xu, Jie [2 ]
Zhan, Yanqiang [3 ]
Tang, Zhouping [1 ]
Zhang, Suming [1 ]
机构：
[1] Huazhong Univ Sci & Technol, Dept Neurol, Tongji Hosp, Tongji Med Coll, Wuhan 430030, Hubei Province, Peoples R China
[2] Capital Med Univ, Beijing Tiantan Hosp, Dept Neurol, Beijing 100050, Peoples R China
[3] Hubei Gen Hosp, Dept Neurol, Wuhan 430030, Hubei Province, Peoples R China
来源：
NEURAL REGENERATION RESEARCH | 2011年 / 6卷 / 01期
基金：
中国国家自然科学基金;
关键词：
induced pluripotent stem cells; mice; microRNA; neural stem cells; HUMAN FIBROBLASTS; GENERATION; INDUCTION; ABSENCE;
D O I：
10.3969/j.issn.1673-5374.2011.01.003
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
microRNA is important for maintaining characteristics of embryonic stem cells, and microRNA302a (MiR-302a) has been shown to exert important effects on cell reprogramming. Therefore, the present study used miR294 and miR302a, in combination with Oct4, to induce mouse neural stem cells (NSCs) into induced pluripotent stem (iPS) cells. Following identification of iPS cells, the effects of microRNA on cell reprogramming were analyzed. Results suggested that reprogramming efficiency with Oct4 + miR-294 + miR-302a was 7-fold greater than Oct4 alone (0.1% vs. 0.014%). The iPS cells were undifferentiated and positive for alkaline phosphatase, SSEA-1, and Oct4. These findings demonstrated that microRNAs play an important role in cell reprogramming and provide a safe and efficient induction system for cellular reprogramming.
引用
收藏
页码：18 / 22
页数：5
相关论文
共 50 条
  • [41] Molecular cloning and production of caprine recombinant Oct4 protein for generation induced pluripotent stem cells
    Singhal, Dinesh K.
    Singhal, Raxita
    Malik, Hruda N.
    Singh, Surender
    Kumar, Sudarshan
    Kaushik, Jai K.
    Mohanty, Ashok K.
    Malakar, Dhruba
    MOLECULAR BIOLOGY REPORTS, 2015, 42 (12) : 1583 - 1591
  • [42] Generation of Induced Pluripotent Stem Cells from Human Cord Blood Using OCT4 and SOX2
    Giorgetti, Alessandra
    Montserrat, Nuria
    Aasen, Trond
    Gonzalez, Federico
    Rodriguez-Piza, Ignacio
    Vassena, Rita
    Raya, Angel
    Boue, Stephanie
    Jose Barrero, Maria
    Aran Corbella, Begona
    Torrabadella, Marta
    Veiga, Anna
    Izpisua Belmonte, Juan Carlos
    CELL STEM CELL, 2009, 5 (04) : 353 - 357
  • [43] cAMP and EPAC Signaling Functionally Replace OCT4 During Induced Pluripotent Stem Cell Reprogramming
    Fritz, Ashley L.
    Adill, Maroof M.
    Mao, Sunnie R.
    Schaffer, David V.
    MOLECULAR THERAPY, 2015, 23 (05) : 952 - 963
  • [44] Molecular cloning and production of caprine recombinant Oct4 protein for generation induced pluripotent stem cells
    Dinesh K. Singhal
    Raxita Singhal
    Hruda N. Malik
    Surender Singh
    Sudarshan Kumar
    Jai K. Kaushik
    Ashok K. Mohanty
    Dhruba Malakar
    Molecular Biology Reports, 2015, 42 : 1583 - 1591
  • [45] Rationale and Methodology of Reprogramming for Generation of Induced Pluripotent Stem Cells and Induced Neural Progenitor Cells
    Tian, Zuojun
    Guo, Fuzheng
    Biswas, Sangita
    Deng, Wenbin
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2016, 17 (04)
  • [46] Effective purification of neural stem cells derived from mouse induced pluripotent stem cells
    Maruyama, Masato
    Houtani, Takeshi
    Trifonov, Stefan
    Kase, Masahiko
    Sugimoto, Tetsuo
    NEUROSCIENCE RESEARCH, 2009, 65 : S93 - S93
  • [47] Reprogramming of Cancer Cells into Induced Pluripotent Stem Cells Questioned
    Bang, Jin Seok
    Choi, Na Young
    Lee, Minseong
    Ko, Kisung
    Park, Yo Seph
    Ko, Kinarm
    INTERNATIONAL JOURNAL OF STEM CELLS, 2019, 12 (03) : 430 - 439
  • [48] Advances in Reprogramming Somatic Cells to Induced Pluripotent Stem Cells
    Minal Patel
    Shuying Yang
    Stem Cell Reviews and Reports, 2010, 6 : 367 - 380
  • [49] Advances in Reprogramming Somatic Cells to Induced Pluripotent Stem Cells
    Patel, Minal
    Yang, Shuying
    STEM CELL REVIEWS AND REPORTS, 2010, 6 (03) : 367 - 380
  • [50] Oct4 differentially regulates chromatin opening and enhancer transcription in pluripotent stem cells
    Xiong, Le
    Tolen, Erik A.
    Choi, Jinmi
    Velychko, Sergiy
    Caizzi, Livia
    Velychko, Taras
    Adachi, Kenjiro
    MacCarthy, Caitlin M.
    Lidschreiber, Michael
    Cramer, Patrick
    Schoeler, Hans R.
    ELIFE, 2022, 11
12345