Genomic Analysis of DNA Double-Strand Break Repair in Escherichia coli

被引：2
|
作者：
Hasan, A. M. Mahedi [1 ]
Azeroglu, Benura [1 ]
Leach, David R. F. [1 ]
机构：
[1] Univ Edinburgh, Sch Biol Sci, Inst Cell Biol, Kings Bldg, Edinburgh, Midlothian, Scotland
来源：
HIGH-DENSITY SEQUENCING APPLICATIONS IN MICROBIAL MOLECULAR GENETICS | 2018年 / 612卷
基金：
英国生物技术与生命科学研究理事会;
关键词：
RECBCD ENZYME; INTERACTIONS INVIVO; BACILLUS SUBTILIS; READ ALIGNMENT; RECA PROTEIN; REPLICATION; CHROMOSOME; ORIGIN; RECOMBINATION; FREQUENCIES;
D O I：
10.1016/bs.mie.2018.09.001
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Counting DNA whole genome sequencing reads is providing new insight into DNA double-strand break repair (DSBR) in the model organism Escherichia coli. We describe the application of RecA chromatin immunoprecipitation coupled to genomic DNA sequencing (RecA-ChIP-seq) and marker frequency analysis (MFA) to analyze the genomic consequences of DSBR. We provide detailed procedures for the preparation of DNA and the analysis of data. We compare different ways of visualizing ChIP data and show that alternative protocols for the preparation of DNA for MFA differentially affect the recovery of branched DNA molecules containing Holliday junctions.
引用
收藏
页码：523 / 554
页数：32
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