Characterization of Japanese flounder karyotype by chromosome bandings and fluorescence in situ hybridization with DNA markers

被引:50
|
作者
Fujiwara, Atushi [1 ]
Fujiwara, Mika
Nishida-Umehara, Chizuko
Abe, Syuiti
Masaoka, Tetsuji
机构
[1] Fisheries Res Agcy, Natl Res Inst Aquaculture, Inland Stn, Tamaki Mie 5190423, Japan
[2] Hokkaido Univ, Div Genome Dynam, Lab Anim Cytogenet, Creat Res Initiat Sousei, Sapporo, Hokkaido 0600810, Japan
[3] Hokkaido Univ, Grad Sch Fisheries Sci, Div Marine Biosci, Lab Breeding Sci, Minato Hakodate 0418611, Japan
关键词
Japanese flounder; chromosome banding; rDNA; telomere; FISH;
D O I
10.1007/s10709-006-9136-z
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
The chromosomes of Japanese flounder, Paralichthys olivaceus, were examined by conventional differential staining methods including G-, Q-, C-, silver (Ag)-, fluorochrome, and replication R-bandings and by fluorescence in situ hybridization (FISH) with 5S and 18S rDNAs and telomeric DNA as probes. Replication R-banding substantially made it possible to identify 24 homologous pairs by their RBG-banding pattern and relative length. Both rDNA loci were mapped to chromosome 1, where 5S and 18S rDNA loci were located at the centromeric region and secondary constriction, respectively. C-banding revealed that both rDNA loci were heterochromatic, and 18S rDNA loci were positive for chromomycin A(3) but negative for 4',6-diamidino-2-phenylindole (DAPI) staining. Telomeric FISH signals were observed at all chromosome ends and at the interstitial region of some chromosomes. The observed results were discussed in relation to the karyotype evolution in the order Pleuronectiformes.
引用
收藏
页码:267 / 274
页数:8
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