Compact detector for proteins based on two-photon excitation of native fluorescence

被引：15

作者：

Paul, UP ^{[1
]}

Li, L ^{[1
]}

Lee, ML ^{[1
]}

Farnsworth, PB ^{[1
]}

机构：

[1] Brigham Young Univ, Dept Chem & Biochem, Provo, UT 84602 USA

来源：

ANALYTICAL CHEMISTRY | 2005年 / 77卷 / 11期

关键词：

D O I：

10.1021/ac048161z

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A compact, inexpensive detector for proteins has been constructed based on two-photon excitation of fluorescence from phenylalanine, tyrosine, and tryptophan. The fluorescence was excited by a solid-state microchip laser operating at 532 nm. Detection limits for phenylalanine, tyrosine, and tryptophan were 62 mu M, 2.0 mu M, and 470 nM, respectively, in a volume of 3 fL. The detection limit for a test protein, bovine serum albumin, was 130 nM.

引用

页码：3690 / 3693

页数：4

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