Sensitive measurement of polyethylene glycol-modified proteins

被引：23
|
作者：
Tsai, N [1 ]
Cheng, TL [1 ]
Roffler, SR [1 ]
机构：
[1] Acad Sinica, Inst Biomed Sci, Taipei, Taiwan
来源：
BIOTECHNIQUES | 2001年 / 30卷 / 02期
关键词：
D O I：
10.2144/01302rr03
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
An IgM monoclonal antibody (AGP3) against polyethylene glycol (PEG) was used to assay PEG-modified proteins by ELISA. Peg-modified beta -glucuronidase could be measured at concentrations as low as 15 ng/mL, corresponding to 750 pg (1.8 fmol) of conjugate. This ELISA should be generally applicable to all PEG-modified proteins because AGP3 binds the backbone of the PEG chain independent of the linker used for PEG attachment.
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页码：396 / +
页数：6
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