Listeria monocytogenes infection and activation of human brain microvascular endothelial cells

被引:50
|
作者
Wilson, SL
Drevets, DA
机构
[1] W Virginia Univ, Hlth Sci Ctr, Dept Med, Morgantown, WV 26506 USA
[2] W Virginia Univ, Hlth Sci Ctr, Dept Microbiol, Morgantown, WV 26506 USA
[3] W Virginia Univ, Hlth Sci Ctr, Dept Immunol, Morgantown, WV 26506 USA
来源
JOURNAL OF INFECTIOUS DISEASES | 1998年 / 178卷 / 06期
关键词
D O I
10.1086/314490
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Listeria monocytogenes invasion of human brain microvascular endothelial cells (BMEC) and its role as a stimulus for endothelial cell activation were studied. Binding and invasion of intact BMEC monolayers were independent of the L. monocytogenes inlAB invasion locus. Cytochalasin D abrogated invasion of BMEC, whereas genistein effected only a 53% decrease in invasion, indicating a requirement for rearrangement of actin microfilaments but less dependence on tyrosine kinase activity. L, monocytogenes stimulated surface expression of E-selectin, ICAM-1, and to a lesser extent, VCAM-1, whereas L. monocytogenes prfA - and Delta hly mutants were severely compromised in this respect. Other experiments showed that BMEC infection stimulated monocyte and neutrophil adhesion and that CD18-mediated binding was the predominant mechanism for neutrophil adhesion to infected BMEC under static conditions. These data suggest that invasion of BMEC is a mechanism for triggering inflammation and leukocyte recruitment into the central nervous system during bacterial meningitis.
引用
收藏
页码:1658 / 1666
页数:9
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