An NMR-based docking model for the physiological transient complex between cytochrome f and cytochrome c6

被引：28
|
作者：
Díaz-Moreno, I
Diaz-Quintana, A
Ubbink, M
De la Rosa, MA
机构：
[1] Univ Sevilla, Inst Bioquim Vegetal & Fotosintesis, Seville 41092, Spain
[2] CSIC, Seville 41092, Spain
[3] Leiden Univ, Leiden Inst Chem, Gorlaeus Labs, NL-2300 RA Leiden, Netherlands
来源：
FEBS LETTERS | 2005年 / 579卷 / 13期
关键词：
cytochrome c(6); cytochrome f; transient interactions;
D O I：
10.1016/j.febslet.2005.04.031
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The physiological transient complex between cytochrome f(Cf) and cytochrome c(6) (Cc(6)) from the cyanobacterium Nostoc sp. PCC 7119 has been analysed by NMR spectroscopy. The binding constant at low ionic strength is 8 +/- 2 mM(-1), and the binding site Of Cc(6) for Cf is localized around its exposed haem edge. On the basis of the experimental data, the resulting docking simulations suggest that Cc(6) binds to Cf in a fashion that is analogous to that of plastocyanin but differs between prokaryotes and eukaryotes. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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页码：2891 / 2896
页数：6
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