Quantitative detection of metallo-β-lactamase of blaIMP-cluster-producing Pseudomonas aeruginosa by real-time polymerase chain reaction with melting curve analysis for rapid diagnosis and treatment of nosocomial infection

被引：7
|
作者：
Motoshima, Maiko [1 ,3 ]
Yanagihara, Katsunori [1 ,3 ]
Yamamoto, Kazuko [2 ,3 ]
Morinaga, Yoshitomo [1 ,3 ]
Matsuda, Junichi [1 ,3 ]
Sugahara, Kazuyuki [1 ,3 ]
Hirakata, Yoichi [1 ,3 ]
Yamada, Yasuaki [1 ,3 ]
Kohno, Shigeru [2 ,3 ]
Kamihira, Shimeru [1 ,3 ]
机构：
[1] Nagasaki Univ, Dept Lab Med, Grad Sch Biomed Sci, Nagasaki 8528501, Japan
[2] Nagasaki Univ, Dept Internal Med 2, Grad Sch Biomed Sci, Nagasaki 8528501, Japan
[3] Nagasaki Univ Hosp, Cent Diagnost Lab, Nagasaki 8528501, Japan
来源：
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2008年 / 61卷 / 02期
关键词：
Pseudomonas aeruginosa; quantitative detection; metallo-beta-lactamase; melting curve analysis; blaIMP; gyrB;
D O I：
10.1016/j.diagmicrobio.2008.01.018
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
In this study, we established the rapid quantitative detection of metallo-beta-lactamase-producing Pseudomonas aeruginosa in clinical isolates and samples using real-time polymerase chain reaction (PCR) targeting gyrB (identification of P. aeruginosa) and blaIMP. The relative sensitivities and specificities of this real-time PCR assay were as follows: 100.0% and 100.0% for clinical isolates, and 100.0% and 98.4% for clinical specimens, respectively. The relative sensitivities and specificities of blaIMP-PCR were 100.0% in both clinical isolates and clinical specimens. The present PCR assay was easily and quickly performed, and it accurately detected P. aeruginosa and metallo-beta-lactamase. (C) 2008 Elsevier Inc. All rights reserved.
引用
收藏
页码：222 / 226
页数：5
相关论文
共 31 条
  • [21] Rapid identification of Bordetella pertussis pertactin gene variants using LightCycler real-time polymerase chain reaction combined with melting curve analysis and gel electrophoresis
    Mäkinen, J
    Viljanen, MK
    Mertsola, J
    Arvilommi, H
    He, QS
    EMERGING INFECTIOUS DISEASES, 2001, 7 (06) : 952 - 958
  • [22] Real-time multiplex polymerase chain reaction with high-resolution melting-curve analysis for the diagnosis of enteric infections associated with diarrheagenic Escherichia coli
    Devi, Thingujam Surbala
    Durairaj, Elantamilan
    Lyngdoh, Wihiwot Valarie
    Duwarah, Sourabh Gohain
    Khyriem, Annie Bakorlin
    Lyngdoh, Clarissa Jane
    INDIAN JOURNAL OF MEDICAL MICROBIOLOGY, 2018, 36 (04) : 547 - 556
  • [23] Application of a real-time fluorescence resonance energy transfer polymerase chain reaction assay with melting curve analysis for the detection of Paragonimus heterotremus eggs in the feces of experimentally infected cats
    Tantrawatpan, Chairat
    Intapan, Pewpan M.
    Thanchomnang, Tongjit
    Sanpool, Oranuch
    Janwan, Penchom
    Lulitanond, Viraphong
    Anamnart, Witthaya
    Maleewong, Wanchai
    JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, 2013, 25 (05) : 620 - 626
  • [24] Detection of angiotensin-converting enzyme insertion/deletion polymorphisms using real-time polymerase chain reaction and melting curve analysis with SYBR Green I on a GeneAmp 5700
    Hiratsuka, M
    Kishikawa, Y
    Narahara, K
    Inoue, T
    Hamdy, SI
    Agatsuma, Y
    Tomioka, Y
    Mizugaki, M
    ANALYTICAL BIOCHEMISTRY, 2001, 289 (02) : 300 - 303
  • [25] Polymorphisms of vascular cell adhesion molecule1 (VCAM1) in polycystic ovary syndrome determined by quantitative real-time polymerase chain reaction and melting curve analysis
    Kanmaz-Ozer, Muge
    Vural, Pervin
    Dogru-Abbasoglu, Semra
    Gedikbasi, Ali
    Cil, Esra
    Karadag, Berrin
    Uysal, Mujdat
    EUROPEAN JOURNAL OF OBSTETRICS & GYNECOLOGY AND REPRODUCTIVE BIOLOGY, 2012, 160 (02) : 174 - 178
  • [26] Assay for the simultaneous detection of the *1C and *1F alleles of the CYP1A2 gene by real-time polymerase chain reaction and melting curve analysis
    Casley, WL
    LeBlanc-Westwood, CA
    PSYCHIATRIC GENETICS, 2006, 16 (02) : 81 - 83
  • [27] RAPID DIAGNOSIS OF THE α-THALASSEMIA-1 SOUTHEAST ASIAN TYPE DELETION USING A SINGLE TUBE REAL-TIME SYBR-POLYMERASE CHAIN REACTION COMBINED WITH DISSOCIATION CURVE ANALYSIS
    Liu, Jing-Zhong
    Xiao, Bai
    Wang, Qing-Tao
    Wang, Li-Rong
    HEMOGLOBIN, 2009, 33 (06) : 546 - 549
  • [28] Detection of Babesia canis vogeli and Hepatozoon canis in canine blood by a single-tube real-time fluorescence resonance energy transfer polymerase chain reaction assay and melting curve analysis
    Kongklieng, Amornmas
    Intapan, Pewpan M.
    Boonmars, Thidarut
    Thanchomnang, Tongjit
    Janwan, Penchom
    Sanpool, Oranuch
    Lulitanond, Viraphong
    Taweethavonsawat, Piyanan
    Chungpivat, Sudchit
    Maleewong, Wanchai
    JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, 2015, 27 (02) : 191 - 195
  • [29] Comparing the diagnostic accuracy of rapid antigen detection tests to real time polymerase chain reaction in the diagnosis of SARS-CoV-2 infection: A systematic review and meta-analysis
    Lee, Jonghoo
    Song, Jae-Uk
    Shim, Sung Ryul
    JOURNAL OF CLINICAL VIROLOGY, 2021, 144
  • [30] A Screening Method for the Detection of the 35S Promoter and the Nopaline Synthase Terminator in Genetically Modified Organisms in a Real-Time Multiplex Polymerase Chain Reaction Using High-Resolution Melting-Curve Analysis
    Akiyama, Hiroshi
    Nakamura, Fumi
    Yamada, Chihiro
    Nakamura, Kosuke
    Nakajima, Osamu
    Kawakami, Hiroshi
    Harikai, Naoki
    Furui, Satoshi
    Kitta, Kazumi
    Teshima, Reiko
    BIOLOGICAL & PHARMACEUTICAL BULLETIN, 2009, 32 (11) : 1824 - 1829
1234