Quantitative detection of metallo-β-lactamase of blaIMP-cluster-producing Pseudomonas aeruginosa by real-time polymerase chain reaction with melting curve analysis for rapid diagnosis and treatment of nosocomial infection

被引：7
|
作者：
Motoshima, Maiko [1 ,3 ]
Yanagihara, Katsunori [1 ,3 ]
Yamamoto, Kazuko [2 ,3 ]
Morinaga, Yoshitomo [1 ,3 ]
Matsuda, Junichi [1 ,3 ]
Sugahara, Kazuyuki [1 ,3 ]
Hirakata, Yoichi [1 ,3 ]
Yamada, Yasuaki [1 ,3 ]
Kohno, Shigeru [2 ,3 ]
Kamihira, Shimeru [1 ,3 ]
机构：
[1] Nagasaki Univ, Dept Lab Med, Grad Sch Biomed Sci, Nagasaki 8528501, Japan
[2] Nagasaki Univ, Dept Internal Med 2, Grad Sch Biomed Sci, Nagasaki 8528501, Japan
[3] Nagasaki Univ Hosp, Cent Diagnost Lab, Nagasaki 8528501, Japan
来源：
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2008年 / 61卷 / 02期
关键词：
Pseudomonas aeruginosa; quantitative detection; metallo-beta-lactamase; melting curve analysis; blaIMP; gyrB;
D O I：
10.1016/j.diagmicrobio.2008.01.018
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
In this study, we established the rapid quantitative detection of metallo-beta-lactamase-producing Pseudomonas aeruginosa in clinical isolates and samples using real-time polymerase chain reaction (PCR) targeting gyrB (identification of P. aeruginosa) and blaIMP. The relative sensitivities and specificities of this real-time PCR assay were as follows: 100.0% and 100.0% for clinical isolates, and 100.0% and 98.4% for clinical specimens, respectively. The relative sensitivities and specificities of blaIMP-PCR were 100.0% in both clinical isolates and clinical specimens. The present PCR assay was easily and quickly performed, and it accurately detected P. aeruginosa and metallo-beta-lactamase. (C) 2008 Elsevier Inc. All rights reserved.
引用
收藏
页码：222 / 226
页数：5
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