Enzymatic hydrolysis of lignin by ligninolytic enzymes and analysis of the hydrolyzed lignin products

被引：100
|
作者：
Zhang, Sitong [1 ,2 ]
Xiao, Jianlong [1 ]
Wang, Gang [1 ,2 ]
Chen, Guang [1 ,2 ]
机构：
[1] Jilin Agr Univ, Coll Life Sci, Changchun, Peoples R China
[2] Minist Educ, Key Lab Straw Biol & Utilizat, Changchun, Peoples R China
来源：
BIORESOURCE TECHNOLOGY | 2020年 / 304卷
基金：
国家重点研发计划;
关键词：
Lignin; Lignin-degrading enzyme; Biodegradation; Chemical bond disruption; Degradation mechanism; DEGRADATION; LACCASE; PRETREATMENT; BACTERIA; ACID; BIOTRANSFORMATION; STRAW; WOOD;
D O I：
10.1016/j.biortech.2020.122975
中图分类号：
S2 [农业工程];
学科分类号：
0828 ;
摘要：
The degradation of alkali lignin was studied using three types of pure enzyme, Lac, LiP, and MnP, using alkali lignin as substrate. The alkali lignin removal rate was found to be 28.98% when Lac, LiP, and MnP were cultured together for alkali lignin degradation. Changes in the structure and composition before and after degradation were characterized by scanning electron microscopy, Fourier-transform infrared spectroscopy, nitrogen adsorption, and gas chromatography-mass spectrometry. The degradation product pathways were analyzed. The enzyme was proven to degrade alkali lignin, resulting in destruction of the alkali lignin structure, ring-opening of the macromolecular benzene ring structure and groups in alkali lignin, and chemical bond cleavage. This study explains the principle of alkali lignin enzymatic hydrolysis and provides a theoretical basis for the biodegradation of lignin.
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页数：6
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