NCAPH Is Required for Proliferation, Migration and Invasion of Non-small-cell Lung Cancer Cells

被引：25
|
作者：
Kim, Byeol [1 ]
Kim, Seok Won [2 ]
Lim, Ji-Yeon [1 ]
Park, Seon-Joo [1 ]
机构：
[1] Chosun Univ, Coll Med, Dept Premed Sci, 309 Philmoon Daero, Gwangju 61452, South Korea
[2] Chosun Univ, Coll Med, Dept Neurosurg, Gwangju, South Korea
来源：
ANTICANCER RESEARCH | 2020年 / 40卷 / 06期
基金：
新加坡国家研究基金会;
关键词：
NCAPH; condensin; chromosome; cell cycle; NSCLC; CONDENSIN-I; TARGETS;
D O I：
10.21873/anticanres.14305
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Background/Aim: Non-structural maintenance of chromosomes condensin I complex subunit H (NCAPH) is implicated in correct chromosome condensation and segregation during mitosis. However, the functional role of NCAPH in the pathogenesis of non-small-cell lung cancer (NSCLC) remains unclear. The aim of this study was to elucidate the role of NCAPH in NSCLC cells. Materials and Methods: A549 and H1299 NSCLC cells were transfected with small-interfering RNA (siRNA) against NCAPH. Subsequently, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, colony-formation assay and flow cytometry analysis were performed to reveal the role of NCAPH in NSCLC cells. In addition, migration and invasion assay were also performed. Results: NCAPH knockdown inhibited cell proliferation, induced cell-cycle arrest at G(2)/M phase, and prevented colony formation, migration and invasion by NSCLC cells. Conclusion: NCAPH is involved in NSCLC progression and development, and may be a potential therapeutic target for NSCLC treatment.
引用
收藏
页码：3239 / 3246
页数：8
相关论文
共 50 条
  • [31] Pyruvate carboxylase is critical for non-small-cell lung cancer proliferation
    Sellers, Katherine
    Fox, Matthew P.
    Bousamra, Michael, II
    Slone, Stephen P.
    Higashi, Richard M.
    Miller, Donald M.
    Wang, Yali
    Yan, Jun
    Yuneva, Mania O.
    Deshpande, Rahul
    Lane, Andrew N.
    Fan, Teresa W. -M.
    JOURNAL OF CLINICAL INVESTIGATION, 2015, 125 (02): : 687 - 698
  • [32] Triptonodiol, a Diterpenoid Extracted from Tripterygium wilfordii, Inhibits the Migration and Invasion of Non-Small-Cell Lung Cancer
    Ni, Xiaochen
    Jiang, Xiaomin
    Yu, Shilong
    Wu, Feng
    Zhou, Jun
    Mao, Defang
    Wang, Haibo
    Liu, Yanqing
    Jin, Feng
    MOLECULES, 2023, 28 (12):
  • [33] Long non-coding RNA DUXAP8 regulates the cell proliferation and invasion of non-small-cell lung cancer
    Yang, Si-Jia
    Weng, Jia-Lu
    Wei, Bin
    Du, Xue-Kui
    OPEN LIFE SCIENCES, 2019, 14 (01): : 201 - 207
  • [34] Non-Small-Cell Lung Cancer
    不详
    GENETIC ENGINEERING & BIOTECHNOLOGY NEWS, 2008, 28 (20): : 61 - 61
  • [35] Non-small-cell lung cancer
    Cesare Gridelli
    Antonio Rossi
    David P. Carbone
    Juliana Guarize
    Niki Karachaliou
    Tony Mok
    Francesco Petrella
    Lorenzo Spaggiari
    Rafael Rosell
    Nature Reviews Disease Primers, 1
  • [36] microRNA-361 targets Wilms' tumor 1 to inhibit the growth, migration and invasion of non-small-cell lung cancer cells
    Yang, Shuxiang
    Zhang, Yingchao
    Zhao, Xin
    Wang, Jingzheng
    Shang, Jianjing
    MOLECULAR MEDICINE REPORTS, 2016, 14 (06) : 5415 - 5421
  • [37] Non-small-cell lung cancer
    Gridelli, Cesare
    Rossi, Antonio
    Carbone, David P.
    Guarize, Juliana
    Karachaliou, Niki
    Mok, Tony
    Petrella, Francesco
    Spaggiari, Lorenzo
    Rosell, Rafael
    NATURE REVIEWS DISEASE PRIMERS, 2015, 1
  • [38] Non-small-cell lung cancer
    不详
    NATURE REVIEWS DISEASE PRIMERS, 2024, 10 (01):
  • [39] Non-small-cell lung cancer
    Nature Reviews Disease Primers, 1 (1)
  • [40] miR-4284 Promotes Cell Proliferation, Migration, and Invasion in Non-Small Cell Lung Cancer Cells and is Associated with Postoperative Prognosis
    Yang, Hanbing
    Zhang, Wenjing
    Luan, Qingxia
    Liu, Yanchao
    CANCER MANAGEMENT AND RESEARCH, 2021, 13 : 5865 - 5872
12345