Analysis of green fluorescent protein bioluminescence in vivo and in vitro using a glow discharge

被引：0
|
作者：
Hernandez, L. [1 ]
Mandujano, L. A. [1 ]
Cuevas, J. [1 ]
Reyes, P. G. [2 ]
Osorio-Gonzalez, D. [1 ]
机构：
[1] Autonomous Univ State Mexico, Fac Sci, Lab Mol Biophys, Toluca 50000, State Mexico, Mexico
[2] Autonomous Univ State Mexico, Fac Sci, Lab Adv Phys, Toluca 50000, State Mexico, Mexico
来源：
15TH LATIN AMERICAN WORKSHOP ON PLASMA PHYSICS (LAWPP 2014) AND 21ST IAEA TM ON RESEARCH USING SMALL FUSION DEVICES (RUSFD) | 2015年 / 591卷
关键词：
EXPRESSION;
D O I：
10.1088/1742-6596/591/1/012068
中图分类号：
O35 [流体力学]; O53 [等离子体物理学];
学科分类号：
070204 ; 080103 ; 080704 ;
摘要：
The discovery of fluorescent proteins has been a revolution in cell biology and related sciences because of their many applications, mainly emphasizing their use as cellular markers. The green fluorescent protein (GFP) is one of the most used as it requires no cofactors to generate fluorescence and retains this property into any organism when it is expressed by recombinant DNA techniques, which is a great advantage. In this work, we analyze the emission spectra of recombinant green fluorescent protein in vivo and in vitro exposed to a glow discharge plasma of nitrogen in order to relate electron temperature to fluorescence intensity.
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页数：4
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