Identification of 18 new transcribed retrotransposons in Schistosoma mansoni

被引：33
|
作者：
DeMarco, R [1 ]
Machado, AA [1 ]
Bisson, AW [1 ]
Verjovski-Almeida, S [1 ]
机构：
[1] Univ Sao Paulo, Inst Quim, Dept Bioquim, Lab Bioinformat, BR-05508900 Sao Paulo, Brazil
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2005年 / 333卷 / 01期
基金：
巴西圣保罗研究基金会;
关键词：
Schistosoma mansoni; phylogenetic analyses; LTR retrotransposon; non-LTR retrotransposon; R2 family retrotransposon; transcribed retrotransposons;
D O I：
10.1016/j.bbrc.2005.05.080
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
This work describes 18 new transcribed retrotransposons of the blood fluke Schistosoma mansoni. Among them, 9 were LTR, 8 non-LTR, and 1 Penelope-like element (PLE) retrotransposon. Sequences were generated by in silico reconstruction using S. mansoni ESTs and transcripts obtained by rapid amplification of cDNA ends, complemented in some cases by sequencing of genomic clones amplified by PCR. A novel element from the ancient R2/R4/CRE transposon group is described for the first time in S. mansoni. In addition, one non-LTR retrotransposon family displays long (40-450 bp) 3'-UTR with at least six different transcribed sequences among the copies, five LTR retrotransposons have abundantly transcribed incomplete copies lacking the sequence segment coding for the reverse transcriptase domain, and four non-LTR retrotransposons code for DNA-binding PHD domains that may give them a differential targeting. These results allow for a comprehensive description of the transcribed retrotransposon diversity of this complex human parasite. (c) 2005 Elsevier Inc. All rights reserved.
引用
收藏
页码：230 / 240
页数：11
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