Localisation of trace metals in metal-accumulating plants using μ-PIXE

被引：17
|
作者：
Siegele, R. [1 ]
Kachenko, A. G. [2 ]
Bhatia, N. P. [1 ]
Wang, Y. D. [3 ]
Ionescu, M. [1 ]
Singh, B. [2 ]
Baker, A. J. M. [3 ]
Cohen, D. D. [1 ]
机构：
[1] Australian Nucl Sci & Technol Org, Menai, NSW 2234, Australia
[2] Univ Sydney, Fac Agr Food & Nat Resources, Sydney, NSW 2006, Australia
[3] Univ Melbourne, Sch Bot, Melbourne, Vic 3010, Australia
来源：
X-RAY SPECTROMETRY | 2008年 / 37卷 / 02期
关键词：
D O I：
10.1002/xrs.1035
中图分类号：
O433 [光谱学];
学科分类号：
0703 ; 070302 ;
摘要：
Particle induced x-ray emission (PIXE) is a very sensitive technique that can quickly and reliably measure a wide range of elements simultaneously with high sensitivity. Using a focused microbeam, elemental distributions can be mapped with high spatial resolution. We demonstrate high-resolution mapping of metals in plant leaves at 5 mu m resolution and its application in detecting sites of metal accumulation in metal-accumulating plant tissues. The importance of biological sample preparation is discussed by direct comparison of freeze-substitution and freeze-drying techniques routinely used in biological sciences. The advantages and limitations of quantitative elemental imaging using these techniques are also discussed. Copyright (C) 2008 John Wiley & Sons, Ltd.
引用
收藏
页码：133 / 136
页数：4
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