Measurement of protein size in concentrated solutions by small angle X-ray scattering

被引：5

作者：

Liu, Jun ^{[1
]}

Li, Zhihong ^{[1
]}

Wei, Yanru ^{[1
]}

Wang, Wenjia ^{[1
]}

Wang, Bing ^{[2
]}

Liang, Hongli ^{[2
]}

Gao, Yuxi ^{[2
]}

机构：

[1] Chinese Acad Sci, Inst High Energy Phys, Beijing Synchrotron Radiat Facil, Beijing 100049, Peoples R China

[2] Chinese Acad Sci, Inst High Energy Phys, CAS Key Lab Biol Effects Nanomat & Nanosafety, Beijing 100049, Peoples R China

来源：

PROTEIN SCIENCE | 2016年 / 25卷 / 08期

关键词：

protein size; inter-particle interference; distance distribution function; small angle X-ray scattering; LIGHT-SCATTERING; SAXS;

D O I：

10.1002/pro.2957

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

By simulations on the distance distribution function (DDF) derived from small angle X-ray scattering (SAXS) theoretical data of a dense monodisperse system, we found a quantitative mathematical correlation between the apparent size of a spherically symmetric (or nearly spherically symmetric) homogenous particle and the concentration of the solution. SAXS experiments on protein solutions of human hemoglobin and horse myoglobin validated the correlation. This gives a new method to determine, from the SAXS DDF, the size of spherically symmetric (or nearly spherically symmetric) particles of a dense monodisperse system, specifically for protein solutions with interference effects.

引用

页码：1385 / 1389

页数：5

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