Roles of FGF-2 and TGF-beta/FGF-2 on differentiation of human mesenchymal stem cells towards nucleus pulposus-like phenotype

被引：21
|
作者：
Zhou, Xiaopeng [1 ]
Tao, Yiqing [1 ]
Wang, Jin [1 ]
Liang, Chengzhen [1 ]
Wang, Jun [1 ]
Li, Hao [1 ]
Chen, Qixin [1 ]
机构：
[1] Zhejiang Univ, Sch Med, Affiliated Hosp 2, Dept Orthoped Surg, Hangzhou 310009, Zhejiang, Peoples R China
来源：
GROWTH FACTORS | 2015年 / 33卷 / 01期
关键词：
Differentiation; fibroblast growth factor-2; mesenchymal stem cells; nucleus pulposus-like phenotype; transforming growth factor-beta; FIBROBLAST-GROWTH-FACTOR; INTERVERTEBRAL DISC REGENERATION; IN-VITRO; SIGNALING PATHWAYS; TRANSPLANTATION THERAPY; ARTICULAR-CARTILAGE; EXPRESSION; PROLIFERATION; ACTIVATION; ERK;
D O I：
10.3109/08977194.2014.969420
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Human mesenchymal stem cells (MSCs) are reported to have the capability of differentiating towards nucleus pulposus (NP)-like phenotype under specific culture conditions. So far, the effects of fibroblast growth factor (FGF)-2 and the cocktail effects of transforming growth factor (TGF)-beta and FGF-2 on MSCs remain unclear. Therefore, we designed this study to clarify these effects. MSCs were cultured in conditioned medium containing FGF-2 or TGF-beta/FGF-2, and compared with basal or TGF-beta medium. The groups with FGF-2 showed the increase of cell proliferation. Functional gene markers and novel NP markers decreased in FGF-2 group, together with functional protein expression. Pho-ERK1/2 and pho-Smad3 differed significantly in the two conditioned groups. All these results suggest FGF-2 promotes MSCs' proliferation, synergistically with TGF-beta. However, FGF-2 plays a negative role in cartilage homeostasis. We also demonstrate that FGF-2 has no positive effect in differentiating MSCs into NP-like cells, but hinders the acceleration effect of TGF-beta.
引用
收藏
页码：23 / 30
页数：8
相关论文
共 50 条
  • [31] Overexpression of Human HMW FGF-2 but Not LMW FGF-2 Reduces the Cytotoxic Effect of Lentiviral Gene Transfer in Human Corneal Endothelial Cells
    Valtink, Monika
    Knels, Lilla
    Stanke, Nicole
    Engelmann, Katrin
    Funk, Richard H. W.
    Lindemann, Dirk
    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2012, 53 (06) : 3207 - 3214
  • [32] A putative role for endogenous FGF-2 in FGF-1 mediated differentiation of human preadipocytes
    Hutley, Louise J.
    Newell, Felicity S.
    Kim, Yu-Hee
    Luo, Xiao
    Widberg, Charlotte H.
    Shurety, Wenda
    Prins, Johannes B.
    Whitehead, Jonathan P.
    MOLECULAR AND CELLULAR ENDOCRINOLOGY, 2011, 339 (1-2) : 165 - 171
  • [33] Differentiation of immortalized human precartilaginous stem cells into nucleus pulposus-like cells
    Wang, Qiong
    Gu, Xiaofeng
    Cheng, Li
    Wang, Junfang
    INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY, 2015, 8 (03): : 2816 - 2822
  • [34] FGF-2 inhibits apoptosis in human teratocarcinoma cells during differentiation on collagen substratum
    Alanko, T
    Tienari, J
    Lehtonen, E
    Saksela, O
    EXPERIMENTAL CELL RESEARCH, 1996, 228 (02) : 306 - 312
  • [35] Transfection of mesenchymal stem cells with the FGF-2 gene improves their survival under hypoxic conditions
    Song, H
    Kwon, K
    Lim, S
    Kang, SM
    Ko, YG
    Xu, Z
    Chung, JH
    Kim, BS
    Lee, H
    Joung, B
    Park, S
    Choi, D
    Jang, Y
    Chung, NS
    Yoo, KJ
    Hwang, KC
    MOLECULES AND CELLS, 2005, 19 (03) : 402 - 407
  • [36] Effects of rAAV-mediated expression of FGF-2 upon the chondrogenesis and proliferation of human mesenchymal stem cells
    Cucchiarini, Magali
    Terwilliger, Ernest
    Graessel, Susanne
    Kohn, Dieter
    Madry, Henning
    HUMAN GENE THERAPY, 2009, 20 (11) : 1487 - 1487
  • [37] ROLE OF HYPOXIA AND GROWTH AND DIFFERENTIATION FACTOR-5 ON DIFFERENTIATION OF HUMAN MESENCHYMAL STEM CELLS TOWARDS INTERVERTEBRAL NUCLEUS PULPOSUS-LIKE CELLS
    Stoyanov, J. V.
    Gantenbein-Ritter, B.
    Bertolo, A.
    Aebli, N.
    Baur, M.
    Alini, M.
    Grad, S.
    EUROPEAN CELLS & MATERIALS, 2011, 21 : 533 - 547
  • [38] Mechanical stress-induced FGF-2 promotes proliferation and consequently induces osteoblast differentiation in mesenchymal stem cells
    Ogura, Hiroyuki
    Nakamura, Takashi
    Ishii, Takenobu
    Saito, Akiko
    Onodera, Shoko
    Yamaguchi, Akira
    Nishii, Yasushi
    Azuma, Toshifumi
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2023, 684
  • [39] Efficient cultivation of neural stem cells with controlled delivery of FGF-2
    Galderisi, U.
    Peluso, G.
    Di Bernardo, G.
    Calarco, A.
    D'Apolito, M.
    Petillo, O.
    Cipollaro, M.
    Fusco, F. R.
    Melone, M. A. B.
    STEM CELL RESEARCH, 2013, 10 (01) : 85 - 94
  • [40] The Role of Endogenous FGF-2 in heterogeneity and pluripotency of human embryonic stem cells.
    Matulka, K.
    Eiselleova, L.
    Kunova, M.
    Hampl, A.
    Sun, Y-M.
    Dvorak, P.
    MOLECULAR BIOLOGY OF THE CELL, 2011, 22
12345