Preparation of blood samples for electron microscopy: The standard protocol

被引：12
|
作者：
Choudhary, Om Prakash [1 ]
Sarkar, Rupan [1 ]
Priyanka [2 ]
Chethan, G. E. [3 ]
Doley, Probal Jyoti [1 ]
Kalita, Pranab Chandra [1 ]
Kalita, Arup [1 ]
机构：
[1] Cent Agr Univ I, Coll Vet Sci & Anim Husb, Dept Vet Anat & Histol, Aizawl 796015, Mizoram, India
[2] Cent Agr Univ I, Coll Vet Sci & Anim Husb, Aizawl 796015, Mizoram, India
[3] Cent Agr Univ I, Coll Vet Sci & Anim Husb, Dept Vet Med, Aizawl 796015, Mizoram, India
来源：
ANNALS OF MEDICINE AND SURGERY | 2021年 / 70卷
关键词：
Blood; Electron microscope; Red blood cells; White blood cells; Platelets; Osmium tetroxide; Phosphate buffer saline; Karnovasky's fixative;
D O I：
10.1016/j.amsu.2021.102895
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
Electron microscopy is a powerful tool to study biological samples at higher magnification. The higher magnifications achieved by the electron microscopes are helpful to the researchers to study surface morphology as well as cellular morphology of the samples. The blood sample surface morphology can be visualized at higher magnification by scanning electron microscope (SEM). For the examination of the blood cells at the cellular level, transmission electron microscopes (TEM) are used. In this article, we have described the step-by-step standard protocol for the preparation of blood samples for electron microscopy. The prepared blood samples can be visualized under SEM and TEM. The obtained electron micrographs of blood cells can be used for differential diagnosis of various diseases at the cellular level.
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页数：4
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