Nested PCR-SSCP assay for the detection of p53 mutations in paraffin wax embedded bone tumours: Improvement of sensitivity and fidelity

被引：0
|
作者：
Wang, LT
Smith, A
Iacopetta, B
Wood, DJ
Papadimitriou, JM
Zheng, MH
机构：
[1] UNIV WESTERN AUSTRALIA,DEPT ORTHOPAED SURG,NEDLANDS,WA 6009,AUSTRALIA
[2] UNIV WESTERN AUSTRALIA,DEPT SURG,NEDLANDS,WA 6009,AUSTRALIA
[3] UNIV WESTERN AUSTRALIA,DEPT PATHOL,NEDLANDS,WA 6009,AUSTRALIA
来源：
JOURNAL OF CLINICAL PATHOLOGY-CLINICAL MOLECULAR PATHOLOGY EDITION | 1996年 / 49卷 / 03期
关键词：
DNA extraction; PCR amplification; SSCP; paraffin wax embedded bone tumour specimens;
D O I：
暂无
中图分类号：
R36 [病理学];
学科分类号：
100104 ;
摘要：
DNA extraction and PCR amplification from paraffin wax embedded bone tumour specimens present several difficulties, firstly, because of the abundant matrix they contain and, secondly, because decalcification often causes degradation of DNA. In this report, comparative studies were carried out to determine the most efficient method for DNA extraction and PCR amplification from such specimens. The results indicated that nested PCR produced appropriate strong reaction products with minimal background contamination. A method for DNA extraction from paraffin wax embedded bone tissue and a nested PCR-SSCP technique have been developed for use in such diagnostic specimens.
引用
收藏
页码：M176 / M178
页数：3
相关论文
共 44 条
  • [21] KI-RAS AND P53 MUTATIONS DETECTED BY PCR-SSCP ANALYSIS IN BARRETTS ASSOCIATED CARCINOMAS
    PORSCHEN, R
    MOLSBERGER, G
    SARBIA, M
    BORCHARD, F
    GASTROENTEROLOGY, 1994, 106 (04) : A429 - A429
  • [22] A SENSITIVE AND HIGH-RESOLUTION METHOD FOR THE DETECTION OF MUTATIONS IN THE P53 GENE USING FLUORESCENCE-BASED PCR-SSCP
    KATSURAGI, K
    CHIBA, W
    MATSUBARA, Y
    IKEDA, S
    UETA, C
    KINOSHITA, M
    BIOMEDICAL RESEARCH-TOKYO, 1995, 16 (04): : 273 - 279
  • [23] CHARACTERIZATION OF 10 HUMAN OSTEOSARCOMAS XENOTRANSPLANTED INTO ATHYMIC NUDE-MICE - SCREENING AND DETECTION OF P53 MUTATIONS BY PCR-SSCP
    LOPEZTERRADA, D
    PEYDROOLAYA, A
    TRICHE, T
    LLOMBARTBOSCH, A
    LABORATORY INVESTIGATION, 1993, 68 (01) : A8 - A8
  • [24] Detection of p53 and K-ras mutations by PCR-SSCP in brain tumors of patients of Instituto Nacional de Cancer, Brazil
    Ribeiro, I
    Teixeira, A
    Oliveira, J
    Camara, L
    Rosas, S
    Martins, G
    Nascimento, P
    Carvalho, MGC
    Alves, G
    17TH INTERNATIONAL CANCER CONGRESS, VOL 1 AND 2, 1998, : 453 - 456
  • [25] Detection of p53 gene mutation by rapid PCR-SSCP and its association with poor survival in breast cancer
    Soong, R
    Iacopetta, BJ
    Harvey, JM
    Sterrett, GF
    Dawkins, HJS
    Hahnel, R
    Robbins, PD
    INTERNATIONAL JOURNAL OF CANCER, 1997, 74 (06) : 642 - 647
  • [26] Higher sensitivity of the PCR-SSCP technique than the immunohistochemical technique for the detection of mutation in the exons 5 and 6 of the p53 gene in breast cancer
    Gonzalez, AD
    Hernandez, JJC
    Hernandez, MMA
    Ramos, M
    Sarmiento, RG
    MEDICINA CLINICA, 1996, 106 (19): : 721 - 724
  • [27] PCR-SSCP a sensitive and rapid method to detect mutations in the P53 tumor suppressor gene of patients with advanced colorectal cancer
    Schmitz, M
    Kayser, I
    Pauly, M
    Lagoda, P
    Tureci, O
    Weber, J
    Kerschen, A
    Ries, F
    Dicato, M
    EUROPEAN JOURNAL OF CANCER, 1995, 31A : 448 - 448
  • [28] A sensitive and high-resolution method for detection of mutations in the p53 gene using multiple fluorescence-based symmetric PCR-SSCP analysis
    Katsuragi, K
    Chiba, W
    Ikeda, S
    Ueta, C
    Kinoshita, M
    BIOMEDICAL RESEARCH-TOKYO, 1997, 18 (01): : 57 - 64
  • [29] PCR-SSCP technique for the detection of mutation in the exons 5 and 6 of the p53 gene in breast cancer. Higher sensitivity than immunohistochemical technique
    Duenas, A
    Cruz, JJ
    Abad, MM
    GonzalezSarmiento, R
    Rodriguez, CA
    Fonseca, E
    Gomez, A
    Martin, G
    Sanchez, P
    Salazar, R
    EUROPEAN JOURNAL OF CANCER, 1997, 33 : 25 - 25
  • [30] Assessment of mutations of Ha- and Ki-ras oncogenes and the p53 suppressor gene in seven malignant mesothelioma patients exposed to asbestos - PCR-SSCP and sequencing analyses of paraffin-embedded primary tumors
    Kitamura, F
    Araki, S
    Tanigawa, T
    Miura, H
    Akabane, H
    Iwasaki, R
    INDUSTRIAL HEALTH, 1998, 36 (01) : 52 - 56
12345