Optimization of Fermentation Parameters for Amylase Synthesis from Aspergillus oryzae through Submerged Fermentation

被引:0
|
作者
Siddalingeshwar, K. G. [1 ]
Sudipta, K. M. [1 ]
Chetan, H. [1 ]
Bhavik, G. [1 ]
Rahul, K. [1 ]
Jitendra, S. [1 ]
Rajanikanth, N. [1 ]
Sushma, K. [1 ]
Swagata, S. [1 ]
Sumana, P. [1 ]
Ranjan, K. [1 ]
Subash, A. [1 ]
Nath, Sourav [1 ]
Dutt, Ankur [1 ]
Mohsin, S. M. [1 ]
Dayananda, K. S. [1 ]
Gopinatha, S. M. [3 ]
Ramya, A. N. [1 ]
Hemalatha, N. B. [2 ]
Rajeshwari, C. P. [2 ]
机构
[1] Padmshree Inst Informat Sci, Dept Studies Microbiol, Bangalore 72, Karnataka, India
[2] Tumkur Univ, Dept Biochem, Tumkur, India
[3] Acharya Inst Technol, Bangalore, Karnataka, India
来源
关键词
Fermentation; Amylase; Aspergillus oryzae; ALPHA-AMYLASE; BACILLUS-AMYLOLIQUEFACIENS;
D O I
暂无
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Studies were under taken on Aspergillus oryzae SCBRD11 isolated from the soil sample using potato dextrose agar and starch used as a substrate to evaluate their ability to produce amylase. The amylase producers detected by the clear zone around the colony by simple plate assay method. Among the fifteen isolates Aspergillus oryzae SCBRD11 is the potential strain. The amylase synthesis were increased their yield after the optimization of fermentation parameters. The optimum pH 4.5, temperature 31 degrees C and inoculum size 1.0 ml. This enzyme was growth
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页码:767 / 771
页数:5
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