Virus-host cell interactions in vaccine production cell lines infected with different human influenza A virus variants: A proteomic approach

被引:27
|
作者
Vester, Diana [1 ]
Rapp, Erdmann [2 ]
Kluge, Sabine [2 ]
Genzel, Yvonne [2 ]
Reichl, Udo [1 ]
机构
[1] Otto von Guericke Univ, D-39106 Magdeburg, Germany
[2] Max Planck Inst Dynam Complex Tech Syst, Magdeburg, Germany
关键词
2-D DIGE; Antiviral response; Cell culture engineering; LC-MS/MS; Influenza A virus; Vaccine production cell lines; ACTIN CYTOSKELETON; NS1; PROTEIN; INTERFERON; CULTURE; VERO; IDENTIFICATION; REPLICATION; APOPTOSIS; TRANSCRIPTION; TRANSPORT;
D O I
10.1016/j.jprot.2010.04.006
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A proteomic approach was used to investigate the dynamic cellular host cell response induced by influenza virus infection in two different vaccine production cell lines, MDCK and Vero. For identification of proteins possibly involved in global host cell response mechanisms and virus-host cell interactions in these producer cell lines, quantitative 2-D DIGE and nanoHPLC-nanoESI-MS/MS analysis were performed. In particular, host cell proteome alterations caused by infection with influenza virus variants showing differences in replication characteristics in MDCK cells were compared. Moreover, the host cell response to virus infection in Vero cells with respect to their deficiency in interferon (IFN) production and the need for virus adaptation to optimize productivity of this cell line were analyzed. Several proteins with differential abundance profiles were identified and Western blot analysis was performed for further confirmation of selected proteins. IFN-induced signal transduction, cytoskeleton remodeling, vesicle transport and proteolysis were the principal pathways that changed in infected MDCK cells. In Vero cells alterations of cell interactions, heat shock and oxidative stress response were detected. The findings will improve understanding of host cell response mechanisms during influenza vaccine production and viral strategies to evade these responses and to replicate efficiently in different cell lines. (C) 2010 Elsevier B.V. All rights reserved.
引用
收藏
页码:1656 / 1669
页数:14
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