Expression and functional role of potassium-chloride cotransporters (KCC) in astrocytes and C6 glioma cells

被引：31
|
作者：
Ringel, Florian [1 ,2 ]
Plesnila, Nikolaus [2 ,3 ]
机构：
[1] Tech Univ Munich, Dept Neurosurg, Klinikum Rechts Isar, D-81675 Munich, Germany
[2] Univ Munich, Med Ctr Grosshadern, Inst Surg Res, Munich, Germany
[3] Univ Munich, Med Ctr Grosshadern, Dept Neurosurg, Munich, Germany
来源：
NEUROSCIENCE LETTERS | 2008年 / 442卷 / 03期
关键词：
KCC; cell volume; regulatory volume decrease; K-Cl-cotransporter; astrocytes; C6; glioma;
D O I：
10.1016/j.neulet.2008.07.017
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
Brain edema formation following brain injury is a serious but still poorly treatable medical condition. The understanding of volume regulation in astrocytes, the main cells involved in the formation of cytotoxic brain edema, is key for the development of novel treatment strategies. This study investigates the role of potassium-chloride cotransporters (KCC) for cell volume regulation in glial cells. PCR revealed the expression of KCC isoforms in a glial cell line (C6) and primary cultured astrocytes. Specific inhibition of KCCs caused glial cell swelling and resulted in a complete inhibition of regulatory volume decrease upon hypotonic medium-induced cell swelling. Therefore, our results show that KCCs play an important role in the maintenance and regulation of cell volume in astrocytes. (c) 2008 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码：219 / 223
页数：5
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