Detection of vanC1 gene transcription in vancomycin-susceptible Enterococcus faecalis

被引：22
|
作者：
de Moura, Tiane Martin [1 ,2 ]
Vaz Cassenego, Ana Paula [1 ,2 ]
Campos, Fabricio Souza [3 ,4 ]
Leal Ribeiro, Andrea Machado [5 ]
Franco, Ana Claudia [1 ,3 ]
d'Azevedo, Pedro Alves [7 ]
Frazzon, Jeverson [6 ]
Guedes Frazzon, Ana Paula [1 ,2 ]
机构：
[1] Univ Fed Rio Grande do Sul, Programa Posgrad Microbiol Agr & Ambiente, Porto Alegre, RS, Brazil
[2] Univ Fed Rio Grande do Sul, Bacteriol Lab, Dept Microbiol, Porto Alegre, RS, Brazil
[3] Univ Fed Rio Grande do Sul, Virol Lab, Dept Microbiol, Inst Ciencias Basicas Saude, Porto Alegre, RS, Brazil
[4] Univ Fed Rio Grande do Sul, Programa Posgrad Ciencias Vet, Porto Alegre, RS, Brazil
[5] Univ Fed Rio Grande do Sul, Fac Agron, Dept Zootecnia, Porto Alegre, RS, Brazil
[6] Univ Fed Rio Grande do Sul, Inst Ciencia & Tecnol Alimentos, Porto Alegre, RS, Brazil
[7] Univ Fed Ciencias Saude Porto Alegre, Lab Cocos Gram Posit, Porto Alegre, RS, Brazil
来源：
MEMORIAS DO INSTITUTO OSWALDO CRUZ | 2013年 / 108卷 / 04期
关键词：
Enterococcus faecalis; vancomycin resistance; vanC gene; horizontal gene transfer; MULTIPLEX PCR; VANC-2/3; GENES; SPECIES LEVEL; D-ALANINE; RESISTANCE; IDENTIFICATION; FOOD; CASSELIFLAVUS; GALLINARUM; SEQUENCE;
D O I：
10.1590/S0074-0276108042013009
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
Here we report the presence and expression levels of the vanC(1) and vanC(2/3) genes in vancomycin-susceptible strains of Enterococcus faecalis. The vanC(1) and vanC2/3 genes were located in the plasmid DNA and on the chromosome, respectively. Specific mRNA of the vanC(1) gene was detected in one of these strains. Additionally, analysis of the vanC gene sequences showed that these genes are related to the vanC genes of Enterococcus gallinarum and Enterococcus casseliflavus. The presence of vanC genes is useful for the identification of E. gallinarum and E. casseliflavus. Moreover, this is the first report of vanC mRNA in E. faecalis.
引用
收藏
页码：453 / 456
页数：4
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